镉暴露诱导的斑马鱼体内金属离子稳态失调  

Cadmium-Induced Disruption of Metal Ion Homeostasis in Zebrafish

作　　者：刘曼雪 师程程 耿柠波[2] 高媛[2] 朱秀华[1] 陈吉平[2] LIU Manxue;SHI Chengcheng;GENG Ningbo;GAO Yuan;ZHU Xiuhua;CHEN Jiping(Department of Environmental Science and Engineering,School of Transportation Engineering,Dalian Jiaotong University,Dalian 116021,China;Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;College of Environmental Science and Engineering,Dalian Maritime University,Dalian 116026,China)

机构地区：[1]大连交通大学交通工程学院环境科学与工程系,大连116021 [2]中国科学院大连化学物理研究所,大连116023 [3]大连海事大学环境科学与工程学院,大连116026

出　　处：《生态毒理学报》2025年第2期337-346,共10页Asian Journal of Ecotoxicology

基　　金：国家重点研发计划项目(2022YFC3105500);中国科学院大连化学物理研究所创新基金(DICP I202219)。

摘　　要：作为具有生物积累性的有毒金属,镉具有潜在的生态风险。本研究以斑马鱼为研究对象,探究了24 h半致死浓度下Cd在斑马鱼体内的累积水平以及Cd暴露后对存活斑马鱼体内多种金属元素稳态的影响。结果表明,暴露组斑马鱼体内Cd浓度水平(7.79μg·g^(-1))显著高于对照组Cd浓度水平(0.55μg·g^(-1));同时,Cd暴露引起斑马鱼体内多种元素稳态失调,与对照组(Mn:1.35μg·g^(-1)、Cu:0.98μg·g^(-1)、Rb:15.43μg·g^(-1))相比,Cd暴露导致Mn(2.5μg·g^(-1))、Cu(2.14μg·g^(-1))、Rb(18.31μg·g^(-1))在斑马鱼体内的水平显著增加,通过激光剥蚀电感耦合等离子体质谱仪(LA-ICP-MS)分析,清晰展示了元素在组织中的分布特征,其中Mn、Cu、K、Rb这4种元素在肝脏和肠内的富集增加;另外,Cd暴露促进Sr在斑马鱼骨骼中的富集增加;Cd的暴露会使Ni的肾脏富集减少。本研究表明Cd暴露会在斑马鱼体内引起来一系列金属离子的稳态失调,因此,对Cd污染进行环境监测和健康风险评价十分必要。Cadmium,a toxic metal with biological accumulation,has the potential for ecological risks.This study used zebrafish as the model organism to investigate the accumulation level of Cd in zebrafish bodies at a semi-lethal concentration of 24 h exposure and the impact of Cd exposure on the steady-state of various metal elements in surviving zebrafish.The results showed that the Cd concentration in the exposed group(7.79μg·g^(-1))was significantly higher than that in the control group(0.55μg·g^(-1)).Meanwhile,Cd exposure disrupted the homeostasis of various elements in zebrafish.Compared to the control group(Mn:1.35μg·g^(-1),Cu:0.98μg·g^(-1),Rb:15.43μg·g^(-1)),Cd exposure significantly increased the levels of Mn(2.5μg·g^(-1)),Cu(2.14μg·g^(-1)),and Rb(18.31μg·g^(-1))in zebrafish in the exposed group.Laser ablation inductively coupled plasma mass spectrometry(LA-ICP-MS)provides excellent elemental tissue distribution profiles,with significant enrichment of Mn,Cu,K,and Rb mainly in the liver and intestines of the zebrafish.Additionally,Cd exposure promoted the enrichment of Sr in zebrafish bones,but reduced the accumulation of Ni in the kidney of zebrafish.This study demonstrated that Cd exposure leads to a series of metal ion homeostasis disturbances in zebrafish.Therefore,environmental monitoring and health risk assessment of Cd pollution are essential.

关 键 词：镉 斑马鱼 稳态失调 金属元素 

分 类 号：X171.5[环境科学与工程—环境科学]