免疫组化联合生物信息学分析信号转导与转录激活因子1在乳腺癌中的表达及与临床病理特征的关系  

Immunohistochemical and bioinformatics analysis of STAT 1 expression in breast cancer and its correlation with clinicopathological features

作　　者：于淼 杨良权 姜茜 YU Miao;YANG Liangquan;JIANG Qian(Department of Breast,Qinhuangdao Maternal and Child Health Hospital,Qinhuangdao 066000,China)

机构地区：[1]秦皇岛市妇幼保健院乳腺科,河北秦皇岛066000

出　　处：《东南大学学报(医学版)》2025年第2期205-211,共7页Journal of Southeast University(Medical Science Edition)

基　　金：秦皇岛市重点研发计划(202301A259)。

摘　　要：目的:探究乳腺癌(BC)中信号转导与转录激活因子1(STAT1)的表达情况,并评估其在BC诊断和预后中的预测价值。方法:分别利用免疫组化和TCGA-BC数据研究STAT1在BC组织中的表达,使用受试者工作特征(ROC)曲线评估STAT1在BC中的诊断价值,通过Kaplan-Meier生存分析和Cox回归分析研究STAT1的表达与BC患者预后的关系。结果:在肾上腺皮质癌(ACC)、膀胱尿路上皮癌(BLCA)、BC等33种癌症类型中,STAT1在大多数肿瘤组织中的表达高于正常组织,呈现上调状态。针对BC进一步分析,从TCGA数据集中获取1113例BC患者样本和113例正常样本进行非配对分析,结果显示,BC组织中STAT1的表达显著高于正常组织(P<0.05)。进行配对分析时,对比TCGA数据库中配对的BC与癌旁正常组织,同样显示BC组织中STAT1的表达显著上调(P<0.05)。随着肿瘤T分期和N分期的增加,STAT1的表达量逐渐增加。ROC曲线分析显示,STAT1在BC诊断中的曲线下面积(AUC)为0.768(基于TCGA数据库mRNA表达)和0.970(基于免疫组化检测蛋白表达),具有较高可靠性。Kaplan-Meier生存分析显示,STAT1高表达与BC患者不良预后相关(mRNA高表达时,P=0.011;蛋白高表达时,P=0.036)。亚组分析表明,高STAT1表达显著影响N0/N1(P=0.005)和T1/T2(P=0.019)分期BC患者的总生存期。免疫组化结果显示,BC组织中STAT1阳性表达45例,阴性表达41例,邻近非肿瘤组织中STAT1阳性表达31例,阴性表达55例(χ^(2)=4.621,P<0.001),进一步验证了STAT1在BC细胞中的促癌作用。临床病理特征分析显示,高STAT1组患者表现出更高的T分期(P=0.033)、N分期(P=0.034)和ER阳性特征(P=0.020)。多因素Cox回归分析结果显示,STAT1高表达是BC患者的独立预后危险因素(P<0.05)。结论:STAT1在BC中的高表达可能参与了肿瘤的发生和发展,并且具有作为BC的潜在诊断和预后标志物的潜力。Objective:To investigate the expression of signal transducer and activator of transcription 1(STAT1)in breast cancer(BC)and evaluate its predictive value in diagnosis and prognosis.Methods:Immunohistochemistry and TCGA-BC data were used to study STAT 1 expression in BC tissues,receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of STAT 1 in BC.In addition,the relationship between STAT 1 expression and the prognosis of BC patients was further investigated by Kaplan-Meier survival analysis and Cox regression analysis.Results:In 33 types of cancers,including adrenocortical carcinoma(ACC),bladder urothelial carcinoma(BLCA),BC,and others,the expression of STAT1 in the tumor tissues of most cancer types was higher than that in normal tissues,showing an upregulated state.Further analysis of BC was conducted by retrieving 1113 BC patient samples and 113 normal samples from the TCGA dataset for unpaired analysis.The results showed that the expression of STAT1 in BC tissues was significantly higher than that in normal tissues(P<0.05).When paired analysis was performed,comparing the paired BC and adjacent normal tissues in the TCGA database,the expression of STAT1 in BC tissues was also significantly upregulated(P<0.05).With the increase in tumor T and N stages,the expression level of STAT1 gradually increased.ROC curve analysis indicated that the area under the curve(AUC)for STAT1 in breast cancer diagnosis was 0.768(based on TCGA database mRNA expression)and 0.970(based on immunohistochemical detection of protein expression),showing high reliability.Kaplan-Meier survival analysis showed that high expression of STAT1 was associated with poor prognosis in BC patients(P=0.011 for high mRNA expression;P=0.036 for high protein expression).Subgroup analysis indicated that high STAT1 expression significantly affected the overall survival of patients with N0/N1(P=0.005)and T1/T2(P=0.019)BC.Immunohistochemical results showed that among breast cancer tissues,45 cases were positive for STAT1 express

关 键 词：乳腺癌 信号转导与转录激活因子1 表达 诊断 预后 

分 类 号：R737.9[医药卫生—肿瘤]