利拉鲁肽抑制高糖环境诱导的人肝星状细胞(LX-2) 增殖作用  

Liraglutide inhibiting the proliferation of high glucose-induced human hepatic stellate cells

作　　者：谢加力 章圣朋 陶云松 XIE Jiali;ZHANG Shengpeng;TAO Yunsong(Department of Pharmacy,Wuhu No.2 People′s Hospital,Wuhu 241000,Anhui,China)

机构地区：[1]芜湖市第二人民医院药剂科,安徽芜湖241000 [2]皖南医学院药学院,安徽芜湖241002

出　　处：《皖南医学院学报》2025年第2期121-124,共4页Journal of Wannan Medical College

基　　金：安徽省高等学校自然科学研究重点项目(2023AH051752);安徽省高等学校优秀青年骨干人才国内访学项目(JNFX2023035)。

摘　　要：目的:研究利拉鲁肽对人肝星状细胞(LX-2)增殖的作用。方法:用不同浓度利拉鲁肽(31.25、62.5、125、250、500 nmol/L)处理高糖诱导的LX-2细胞,采用CCK-8法检测细胞活力,流式细胞术分析细胞凋亡率,免疫印迹法检测LX-2细胞内肝纤维化(HF)标志蛋白CollagenⅠ和α-SMA的表达水平。结果:CCK-8实验结果显示,高糖可诱导LX-2细胞增殖(P<0.01),利拉鲁肽作用12、24、48 h可降低高糖环境下LX-2细胞增殖率(P<0.05);流式细胞术检测结果证明利拉鲁肽可诱导细胞发生凋亡(P<0.05);免疫印迹结果表明利拉鲁肽可下调LX-2细胞中CollagenⅠ和α-SMA蛋白表达(P<0.05)。结论:利拉鲁肽可减弱高糖诱导的LX-2活化,增加其凋亡,抑制HF标志物表达。Objective:To observe the effects of liraglutide on human hepatic stellate cells(LX-2 cells)under high glucose conditions.Methods:LX-2 cells induced by high glucose were treated with different dose of liraglutide(31.25,62.5,125,250 and 500 nmol/L,respectively).CCK-8 assay,flow cytometry and Western blot were used respectively to measure the cell viability,the apoptosis rates and the expression levels of fibrosis markers collagen I andα-SMA in LX-2 cells.Results:CCK-8 assay indicated that high glucose promoted the proliferation of LX-2 cells(P<0.01),yet the viability of LX-2 cells was reduced after treatment with liraglutide for 12,24 and 48 hours(P<0.05).The results of the flow cytometry assay demonstrated that liraglutide was able to induce apoptosis in the cells(P<0.05),and Western blotting revealed that liraglutide led to inhibited expression of collagen I andα-SMA proteins in LX-2(P<0.05).Conclusion:Liraglutide may attenuate high glucose-induced activation of LX-2 cells,promote apoptosis,and suppress expression of hepatic fibrosis markers.

关 键 词：肝纤维化 利拉鲁肽 肝脏星状细胞 

分 类 号：R575.2[医药卫生—消化系统] R587.1[医药卫生—内科学]