MLH1、MSH在胃癌组织中的表达分析  

Expression analysis of MLH1 and MSH in gastric cancer tissue

作　　者：黄一鸣 张丽娜 HUANG Yi-ming;ZHANG Li-na(Digestive Endoscopy Center of Anxi Hospital,Quanzhou 362400,China)

机构地区：[1]泉州市安溪县医院消化内镜中心,362400

出　　处：《中国现代药物应用》2025年第7期68-71,共4页Chinese Journal of Modern Drug Application

摘　　要：目的分析MLH1、MSH在胃癌组织中的表达情况。方法选取100例胃癌患者,采用免疫组织化学法(IHC)-链霉菌亲和素-过氧化物酶连结法(SP)检测患者胃癌组织中MLH1、MSH水平,根据检测结果分为高频微卫星不稳定(MSI-H)型胃癌患者(30例)和低频微卫星不稳定或微卫星稳定(MSI-L/MSS)型胃癌患者(70例)。比较MSI-H型胃癌患者与MSI-L/MSS型胃癌患者的临床病理特征、4年生存率。结果MSI-H型胃癌患者的淋巴结转移率6.67%(2/30)和脉管侵犯率33.33%(10/30)低于MSI-L/MSS型胃癌患者的25.71%(18/70)、68.57%(48/70)(P<0.05)。MSI-H型胃癌患者与MSI-L/MSS型胃癌患者的性别、年龄、Borramn分型、Lauren分型、分化程度、浸润深度和远处转移情况比较没有显著差异(P>0.05)。MSI-H型胃癌患者的4年生存率高于MSI-L/MSS型胃癌患者(86.67%VS 64.29%)(χ^(2)=5.109,P=0.024<0.05)。结论MLH1和MSH在胃癌组织表达分析中,不仅与胃癌的淋巴结转移和脉管侵犯相关,还与患者的预后和生存情况有关。Objective To analyze the expression of MLH1 and MSH in gastric cancer tissue.Methods 100 gastric cancer patients were selected.The levels of MLH1 and MSH in gastric cancer tissues were detected using immunohistochemistry(IHC)-streptavidin-peroxidase(SP)method.Based on the results,they were divided into high-frequency microsatellite instability(MSI-H)group and low-frequency microsatellite instability or microsatellite stability(MSI-L/MSS)group.The clinicopathologic characteristics,4-year survival rate of patients with MSI-H type gastric cancer and patients with MSI-L/MSS type gastric cancer were compared.Results The lymph node metastasis rate of MSI-H type gastric cancer patients was 6.67%(2/30)and the vascular invasion rate was 33.33%(10/30),which were lower than 25.71%(18/70)and 68.57%(48/70)of patients with MSI-L/MSS type gastric cancer(P<0.05).There were no significant differences in gender,age,Borramn classification,Lauren classification,differentiation degree,depth of invasion and distant metastasis between MSI-H type gastric cancer patients and MSI-L/MSS type gastric cancer patients(P>0.05).The 4-year survival rate of patients with MSI-H type gastric cancer was higher than those of patients with MSI-L/MSS type gastric cancer(86.67%VS 64.29%)(χ^(2)=5.109,P=0.024<0.05).Conclusion The expression analysis of MLH1 and MSH in gastric cancer tissue is not only correlated with lymph node metastasis and vascular invasion of gastric cancer,but also with the prognosis and survival of patients.

关 键 词：MLH1 MSH 胃癌 胃癌组织 表达分析 淋巴结转移 脉管侵犯 

分 类 号：R735.2[医药卫生—肿瘤]