新型四元氮桥环内酯类活性物质antifungalmycin N2对人宫颈癌Hela细胞的增殖抑制和诱导凋亡作用  

Proliferation inhibition and apoptosis induction of a novel structurally N-heterocyclic bioactive compound antifungalmycin N2 against human cervical cancer Hela cells

作　　者：姚亮[1] 王丽君[1] YAO Liang;WANG Li-jun(Oncology Department,Jiangxi Maternal and Child Health Hospital,Nanchang 330006,China)

机构地区：[1]江西省妇幼保健院肿瘤科,330006

出　　处：《中国现代药物应用》2025年第7期171-175,共5页Chinese Journal of Modern Drug Application

基　　金：江西省卫生健康委普通科技计划(项目编号:202130759)。

摘　　要：目的探究链霉菌N2发酵所产新型四元氮桥环内酯类活性物质antifungalmycin N2对人宫颈癌Hela细胞的增殖抑制和诱导凋亡效果。方法分别利用细胞增殖四唑盐(MTT)检测和流式细胞仪分析,探究antifungalmycin N2对Hela细胞增殖、凋亡和细胞周期分步的影响。结果氮桥环内酯类化合物antifungalmycin N2对人宫颈癌Hela细胞具有有效的细胞毒性,其半抑制浓度(IC50)值为15.37μg/ml。人宫颈癌细胞Hela经不同浓度(5、10、20μg/ml)的氮桥环内酯类化合物antifungalmycinN2处理24 h后,空白对照组、(5、10、20μg/ml)处理组、阳性对照组细胞凋亡率分别为(4.84±1.2)%、(11.09±2.1)%、(24.23±3.9)%、(31.46±5.4)%、(54.26±8.0)%。与空白对照组比较,氮桥环内酯类化合物antifungalmycin N2处理后,细胞凋亡明显增加,差异均有统计学意义(P<0.05),且具有浓度依赖性。经antifungalmycin N2对Hela细胞呈现剂量依赖性的诱导凋亡作用,促使其阻滞在G1期,使进入S期的细胞减少。结论新型四元氮桥环内酯类活性物质antifungalmycin N2对人宫颈癌Hela细胞具有显著的增殖抑制和诱导凋亡作用。Objective To investigate the proliferation inhibition and apoptosis induction of antifungalmycin N2(a novel structurally N-heterocyclic bioactive compound produced by Streptomyces sp.strain N2)against human cervical carcinoma Hela cells.Methods The effect of antifungalmycin N2 on the cell proliferation,apoptosis,and cell cycle progression of Hela cells was investigated using methyl thiazolyl tetrazolium(MTT)assay and flow cytometry analysis.Results Antifungalmycin N2 showed an effective cytotoxicity on Hela cells,with a 50%concentration of inhibition(IC50)value of 15.37μg/ml.After human cervical cancer cells Hela were treated with different concentrations(5,10,20μg/ml)of antifungalmycin N2 for 24 h,the apoptosis rates of the blank control group(5,10,20μg/ml),the treated group,and the positive control group were(4.84±1.2)%,(11.09±2.1)%,(24.23±3.9)%,(31.46±5.4)%,and(54.26±8.0)%.Compared with the blank control group,apoptosis increased significantly after antifungalmycin N2 treatment,and the differences were all statistically significant(P<0.05),and in a concentration-dependent manner.Furthermore,antifungalmycin N2 exhibited a dose-dependent induction of apoptosis on Hela cells through an appreciable cell cycle arrest at G1 phase,thus reducing the number of Hela cells entering the S phase.Conclusion The novel structurally N-heterocyclic bioactive compound antifungalmycin N2 has significant effects on the proliferation inhibition and apoptosis induction of human cervical cancer Hela cells.

关 键 词：Antifungalmycin N2 新型四元氮桥环内酯类活性物质 增殖抑制 细胞周期 细胞凋亡 人宫颈癌HELA细胞 

分 类 号：R737.33[医药卫生—肿瘤]