沉默信息调节因子1调控成骨成血管功能促进颌骨缺损愈合的实验研究  

Promotion of mandibular defect healing through the regulation of osteogenic and angiogenic functions by sirtuin 1

作　　者：刘志凯 刘航航[2] 刘士博 李帛伦 刘瑶 罗恩[1] Liu Zhikai;Liu Hanghang;Liu Shibo;Li Boun;Liu Yao;Luo En(State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Dept.of Orthognathic and Joint Surgery,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China;State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clini‐cal Research Center for Oral Diseases&Dept.of Emergency,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China)

机构地区：[1]口腔疾病防治全国重点实验室、国家口腔医学中心、国家口腔疾病临床医学研究中心、四川大学华西口腔医院正颌及关节外科,成都610041 [2]口腔疾病防治全国重点实验室、国家口腔医学中心、国家口腔疾病临床医学研究中心、四川大学华西口腔医院急诊科,成都610041

出　　处：《国际口腔医学杂志》2025年第3期349-357,共9页International Journal of Stomatology

基　　金：国家自然科学基金面上项目(82370932);四川省重点研发项目(2024YFFK0204);四川省自然科学基金青年项目(2023-NSFSC1512)。

摘　　要：目的 探究沉默信息调节因子1 (SIRT1)在体内外条件下对小鼠成骨成血管功能及颌骨缺损愈合的影响。方法 使用SIRT1特异性激活剂及抑制剂干预小鼠胚胎前体成骨细胞(MC3T3-E1)及小鼠颌骨缺损,采用细胞计数试剂(CCK-8)、实时荧光定量聚合酶链反应、蛋白免疫印迹、碱性磷酸酶(ALP)染色、免疫荧光染色等多种方式,研究SIRT1对MC3T3-E1细胞成骨成血管因子表达、小鼠颌骨缺损愈合及颌骨缺损成骨成血管功能的影响。结果 细胞实验中SIRT1激活时可促进MC3T3-E1细胞成骨成血管因子表达和ALP活性;动物实验中SIRT1激活可促进颌骨缺损的愈合,同时增强颌骨缺损区域成骨成血管功能;抑制SIRT1活性时则会抑制上述过程。结论 SIRT1可通过调控小鼠颌骨成骨成血管功能促进颌骨缺损的愈合过程。Objective This study aims to investigate the effect of sirtuin 1(SIRT1)on osteogenic and angiogenic func‐tions in mice under in vivo and in vitro conditions,as well as its effect on mandibular defect healing.Methods SIRT1 ac‐tivators and inhibitors were used to intervene in MC3T3-E1 cells and mandibular defects in mice.Various methods,in‐cluding cell counting kit-8(CCK-8)assay,real-time quantitative polymerase chain reaction,Western blot,alkaline phos‐phatase staining,and immunofluorescence staining,were employed to study the influence of SIRT1 on the expres‐sion of osteogenic and angiogenic factors in MC3T3-E1 cells,as well as the healing and osteogenic and angio‐genic functions of mandibular defects in mice.Results In in vitro experiments,the activation of SIRT1 promo-ted the expression of osteogenic and angiogenic factors in MC3T3-E1 cells.In in vivo experiments,SIRT1 activation facilitated the hea-ling of mandibular defects and enhanced the osteogenic and angiogenic functions of the mandibular defects.Conversely,the inhibition of SIRT1 activity sup‐pressed the aforementioned processes.Conclusion SIRT1 can promote the healing of mandibular defects by regulating the osteogenic and angiogenic functions in mice.

关 键 词：沉默信息调节因子1 血管生成 颌骨缺损 骨再生 

分 类 号：R782[医药卫生—口腔医学]