支链氨基酸转氨酶1在口腔鳞状细胞癌中的表达及其功能研究  

Expression and function of branch-chain amino acid transaminase 1 in oral squamous cell carcinoma

作　　者：李熠洁 原振英 李明[2] Li Yijie;Yuan Zhenying;Li Ming(Dept.of Dental Aesthetics,Changsha Stomatological Hospital,Changsha 410000,China;Xiangya School/Hospital of Stomatology,Central South University,Key Laboratory of Oral Health Research in Hunan Province,Changsha 410000,China)

机构地区：[1]长沙市口腔医院牙体美容科,长沙410000 [2]中南大学湘雅口腔医(学)院、口腔健康研究湖南省重点实验室,长沙410000

出　　处：《国际口腔医学杂志》2025年第3期358-365,共8页International Journal of Stomatology

基　　金：湖南省重点研发项目(2022SK2052)。

摘　　要：目的 分析支链氨基酸转氨酶1 (BCAT1)在口腔鳞状细胞癌(OSCC)中的表达及生物功能。方法 采用生物信息学分析BCAT1在头颈部鳞状细胞癌(HNSC)患者中的表达、与患者预后的关系。随后,选取2021—2022年在中南大学湘雅口腔医院口腔颌面外科确诊的40名OSCC患者,检测癌及癌旁组织中BCAT1基因表达。分析人OSCC细胞系和正常口腔角质细胞中BCAT1基因表达。质粒过表达或敲低BCAT1基因后,采用细胞增殖、划痕和侵袭实验分别检测人OSCC细胞增殖、迁移和侵袭能力改变。结果 BCAT1在the Cancer Genome Atlas(TCGA)数据库中HNSC患者癌组织中高表达,且是患者的独立预后因素之一和潜在诊断标志物。细胞实验结果表明BCAT1在人OSCC组织和细胞系中表达上调,并调控OSCC细胞增殖、迁移和侵袭。结论 BCAT1基因是人OSCC的潜在治疗靶点。Objective This study aimed to analyze the expression and biological function of branch-chain amino acid transaminase 1(BCAT1)in oral squamous cell carcinoma(OSCC).Methods The expression of BCAT1 in head and neck squamous cell carcinoma(HNSC)patients and its relationship with prognosis were analyzed by bioinformatics.To detect BCAT1 gene expression in tumor and adjacent tissues,we selected patients with OSCC diagnosed in the Depart‐ment of Oral and Maxillofacial Surgery,Xiangya Stomatology Hospital,Central South University from 2021 to 2022.BCAT1 gene expression in human OSCC cell line and normal oral keratinocytes was analyzed.After plasmid overexpres‐sion or knockdown of BCAT1 gene,the proliferation,migration,and invasion ability of human OSCC cells were detected by cell proliferation,wound-healing,and invasion assays,respectively.Results BCAT1 was highly expressed in cancer tissues of HNSC patients in the Cancer Genome Atlas(TCGA)database and was one of the independent prognostic fac‐tors and potential diagnostic markers of patients.Results of cellular assays showed that BCAT1 expression was upregula-ted in human OSCC tissues or cell lines,thereby regulating the proliferation,migration,and invasion of OSCC cells.Con-clusion BCAT1 gene is a potential therapeutic target for human OSCC.

关 键 词：支链氨基酸转氨酶1 口腔鳞状细胞癌 头颈鳞状细胞癌 增殖 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]