玉米穗部叶片叶型结构性状全基因组关联分析  

Genome wide association analysis of leaf architecture traits in ear leaves of maize

作　　者：于从超 许家伟 张嘉月 李琛 辜源 李昊 刘显君[1] 李春翔 张林[1] YU Congchao;XU Jiawei;ZHANG Jiayue;LI Chen;GU Yuan;LI Hao;LIU(Xianjun,LI Chunxiang,ZHANG Lin(School of Agriculture,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学农学院,哈尔滨150030

出　　处：《东北农业大学学报》2025年第1期12-20,共9页Journal of Northeast Agricultural University

基　　金：黑龙江省重点研发计划项目(GA21D005)。

摘　　要：玉米穗部叶片(穗上叶、穗位叶、穗下叶)叶型结构是影响株型的重要性状,对产量起决定性作用。以294份叶型结构不同的玉米自交系组成的自然群体为试验材料,通过调查计算穗部叶片叶夹角和叶向值等表型性状,结合5850982个SNPs标记进行全基因组关联分析(GWAS)。结果表明:供试材料穗部叶片叶型结构性状差异显著,表现出典型的数量性状特点。检测出极显著相关SNP位点570个(P<1.00×10^(-5)),基于与3个及以上性状显著相关的11个SNP位点预测到11个候选基因,通过功能注释和GO富集分析,筛选出3个可能与玉米穗部叶片叶型结构相关的候选基因(Zm00001d026067、Zm00001d026069和Zm00001d051125),主要参与植物信号转导、氧化还原酶活性和超氧化物代谢通路;推测通过激素信号转导和超氧化物代谢途径,影响细胞分裂速度与增殖方式,进而影响玉米穗部叶片叶夹角和叶向值。qRT-PCR结果表明,3个候选基因在叶型结构差异极显著的4个玉米自交系中均差异表达。The leaf architecture of maize ear leaves(upper ear leaves,ear position leaves and lower ear leaves)is an important character affecting plant type and plays a decisive role in yield formation.In this study,294 maize inbred lines with different leaf architecture were used as experimental materials.Phenotypic traits such as ear leaf angle and leaf orientation value were calculated by investigation,combined with 5850982 SNPs markers for genome wide association analysis(GWAS).The results showed that there were significant differences in leaf architecture of panicle leaves,which showed typical quantitative characteristics.A total of 570 SNPs(P<1.00×10^(-5))were detected,and 11 candidate genes were predicted based on 11 SNPS significantly associated with three or more traits.Through functional annotation and GO enrichment analysis,three candidate genes(Zm00001d026067,Zm00001d026069 and Zm00001d051125),which might be related to the leaf architecture of maize ear leaves,were mainly involved in plant signal transduction,redoxase activity and superoxide metabolism pathway.It was speculated that the hormone signal transduction pathway and superoxide metabolism pathway affected the cell division rate and proliferation mode,and then affected the leaf angle or leaf orientation value of maize ear leaves.The qRT-PCR analysis revealed that the three candidate genes exhibited differential expression patterns across the four maize inbred lines,which displayed significant variations in leaf structure.

关 键 词：玉米 穗部叶片 叶型 全基因组关联分析 候选基因 

分 类 号：S513[农业科学—作物学]