The effects of diazepam on sleep depend on the photoperiod  

作　　者：Maria Panagiotou Johanna H.Meijer Tom Deboer 

机构地区：[1]Laboratory for Neurophysiology,Department of Cell and Chemical Biology,Leiden University,Medical Centre,Leiden,2333,ZC,The Netherlands [2]Erasmus University College,Erasmus School of Social and Behavioural Sciences,Life Sciences Department,Erasmus University Rotterdam,Rotterdam,3011,HP,the Netherlands

出　　处：《Acta Pharmacologica Sinica》2025年第4期892-903,共12页中国药理学报(英文版)

基　　金：supported by a grant from the Dutch Technology Foundation(STW);the Velux Stiftung(Zurich,Switzerland number 1289)to TD.

摘　　要：Daylength(i.e.,photoperiod)provides essential information for seasonal adaptations of organisms.Earlier studies have demonstrated that photoperiod influences sleep in several species.Notably,photoperiod can change the excitatory/inhibitory balance in the brain,with long photoperiod exhibiting increasedγ-aminobutyric-acid(GABA)-mediated excitation.In this study,we first investigated whether different photoperiods influence sleep and the sleep electroencephalogram(EEG)in mice,and,subsequently,whether these photoperiods alter GABAergic functioning by treating mice with diazepam(3 mg/kg,i.p.).EEG and electromyogram(EMG)recordings were conducted in mice well-adapted to long or short photoperiod(16:8 vs.8:16 light-dark cycle)in baseline conditions,after 4-h sleep deprivation,and following diazepam administration.Different photoperiods led to a redistribution of sleep and wakefulness in 24-h albeit without altering the overall amount of vigilance states;during darkness,mice exposed to the long photoperiod were more awake and showed very little rapid-eye-movement(REM)sleep compared to the short photoperiod.Furthermore,an overall lower EEG power density,across all vigilance states,was found in the long compared to short photoperiod.After diazepam treatment,slow-wave-activity(SWA)in NREM sleep was suppressed independent of the photoperiod.However,following diazepam administration,mice showed more REM sleep in the short photoperiod,and increased EEG power density in the slower frequencies(2.5–7 Hz),during wakefulness in the long photoperiod.These results demonstrate that photoperiod can affect the diazepam-induced changes on sleep architecture and EEG,suggesting that treatments with GABA_(A)agonists exert dissimilar effects depending on the photoperiod.Future studies are warranted to explore potential photoperiod effects in humans which could have consequences for the treatment of anxiety and sleep disturbances.

关 键 词：SLEEP PHOTOPERIOD EEG slow-wave-activity diazepam GABA 

分 类 号：R338.63[医药卫生—人体生理学]