HMGN5在肝癌组织中的表达及对肝癌细胞生长的影响  

Expression of HMGN5 in hepatocellular carcinoma and its influence on the growth of hepatocellular carcinoma

作　　者：任守雷 李鸿春 曹文丽 孙继泽 赵建文 Ren Shoulei;Li Hongchun;Cao Wenli;Sun Jize;Zhao Jianwen(Oncology Department,Sunshine Union Hospital,Weifang 261205,China;Neurology Department,Sunshine Union Hospital,Weifang 261205,China)

机构地区：[1]山东省潍坊市阳光融和医院肿瘤二科,潍坊261205 [2]山东省潍坊市阳光融和医院神经内科,潍坊261205

出　　处：《中国综合临床》2025年第2期105-110,共6页Clinical Medicine of China

基　　金：山东省医药卫生科技发展计划项目(202202080185)。

摘　　要：目的探讨高迁移率核小体结合蛋白5(high mobility group nucleosome-binding protein,HMGN5)在肝癌组织及肝癌HepG2细胞中的表达及对肝癌HepG2细胞生长的影响。方法选取潍坊市阳光融和医院2017年1月至2020年6月行手术切除并经术后病理确诊的肝细胞癌患者70例,收集其临床和病理资料,以及手术切除的肝癌组织和癌旁正常肝组织,记录术后生存时间。应用免疫组织化学方法检测肝癌组织和癌旁正常肝组织中HMGN5蛋白的表达情况,比较HMGN5蛋白的表达情况,以及不同临床特征肝细胞癌患者肝癌组织中HMGN5蛋白的阳性表达率,分析HMGN5表达与肝细胞癌患者预后的关系。应用实时荧光定量聚合酶链反应法检测肝癌HepG2细胞和肝HL-7702细胞中HMGN5 mRNA的表达情况。用HMGN5短发夹RNA转染HepG2细胞后,通过细胞增殖实验评估敲除HMGN5基因对肝癌HepG2细胞增殖的抑制作用,使用流式细胞仪分析敲除HMGN5基因对肝癌HepG2细胞凋亡情况的影响。呈正态分布的计量资料以x¯±s表示,组间比较采用两独立样本t检验;计数资料以例(%)表示,组间比较采用χ^(2)检验;患者生存分析采用Kaplan-Meier法。结果免疫组织化学染色结果显示,HMGN5在肝癌组织中的染色很强,而在癌旁正常肝组织中的染色很弱。70例肝细胞癌患者手术切除的肝癌组织中HMGN5蛋白表达阳性48例,病理分级3级+4级以及临床分期Ⅲ期+Ⅳ期肝细胞癌患者肝癌组织中HMGN5蛋白阳性表达率分别高于1级+2级、Ⅰ期+Ⅱ期患者[80.0%(28/35)比57.1%(20/35),80.6%(29/36)比55.9%(19/34)],差异均有统计学意义(χ^(2)值分别为4.24、4.94,P值分别为0.039、0.026)。HMGN5阳性表达组患者的累积生存率与阴性表达组比较差异无统计学意义(χ^(2)=3.81,P=0.051)。肝癌HepG2细胞中HMGN5 mRNA的表达高于正常人肝HL-7702细胞[(4.51±0.45)比(1.35±0.27)],差异有统计学意义(t=10.43,P<0.001)。HMGN5基因被敲除24、48、72 h后,�ObjectiveTo investigate the affection of high mobility group nucleosome-binding protein 5(HMGN5)gene in the hepatocellular cancer tissue and HepG2 cell and its function in the growth of HepG2 cell.Methods70 Patients with hepatocellular carcinoma who under-went surgical resection and were confirmed by postoperative pathology in Sunshine Union Hospital of Weifang City from January 2017 to June 2020 were selected.Their clinical and pathological data,surgical resection of liver cancer tissue and adjacent normal liver tissue were collected,their survival time were also recorded.The contents of HMGN5 protein in the hepatocellular cancer tissues and adjacent normal liver tissues of 70 patients were detected by immunohistochemistry.Compare the expression of HMGN5 protein in liver cancer tissue and normal liver tissue and the positive expression rate of HMGN5 protein in liver cancer tissues of hepatocellular carcinoma patients with different clinical characteristics,to analyze the relationship between HMGN5 expression and prognosis of patients with hepatocellular carcinoma.The mRNA contents of HMGN5 gene in HepG2 cells and HL-7702 cells were determined by quantitative real time polymerase chain reaction(qRT-PCR)method.After transfectHepG2 cells with HMGN5 shRNA,the proliferation ability of HepG2 cells were evaluated by cell proliferation assay and the cell apoptosis was analyzed by flow cytometry.Measurement data with normal distribution were expressed as±s,two independent samples t test was used for comparison between groups.Counting data was expressed as n(%),χ^(2) test was used for comparison between groups.Survival analysis of patients was performed by Kaplan-Meier method.ResultsImmunohistochemical tests showed that HMGN5 staining is strong in liver cancer tissue,but weak in normal liver tissue.HMGN5 protein expression was positive in 48 of 70 patients with hepatocellular carcinoma.The positive expression rate of HMGN5 protein in hepatocellular carcinoma tissues of patients with pathological grade 3+4 and clinical s

关 键 词：肝细胞癌 高迁移率核小体结合蛋白5 HEPG2细胞 肝HL-7702细胞 增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]