荧光探针对植物组织乙烯释放的监测及在白熟期番茄中的应用  

Monitoring of Ethylene Release from Plant Tissues by Fluorescent Probe and Application in White Ripe Tomato

作　　者：杨倩 屠鹏[1] 郝振芳 崔彦君[1] 边红霞[1] YANG Qian;TU Peng;HAO Zhenfang;CUI Yanjun;BIAN Hongxia(College of Science,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学理学院,兰州730070

出　　处：《高等学校化学学报》2025年第5期29-36,共8页Chemical Journal of Chinese Universities

基　　金：甘肃省自然科学基金(批准号:22JR5RA866)资助.

摘　　要：以酪蛋白为载体,将第一代Grubbs催化剂与6-甲氧基-2-萘乙烯相结合的荧光探针(P3)包覆在蛋白疏水袋中制成水溶性荧光探针(C-E-P).对P3的性能测试表明,其对乙烯表现出良好的线性关系、快速的响应时间和高选择性.将P3包覆于酪蛋白制成的水溶性探针C-E-P能够实现对苹果、苦水玫瑰和芦荟组织乙烯释放的荧光成像,并可用于白熟期番茄成熟过程中组织的乙烯变化监测,发现种子的乙烯释放峰值先于果皮出现.本文开发的C-E-P探针能够有效捕捉植物组织中乙烯的释放信息,为农业生产中果实成熟和植物健康监测提供了一种有效的工具.The quantification of ethylene can be achieved through the change of fluorescence signal,but there are few reports about the real-time monitoring of ethylene levels in plant tissues.Developing environmentally friendly and biocompatible chemical-based fluorescent probes is one of the ways to achieve plant tissue imaging.This article uses casein as a carrier to create a water-soluble fluorescent probe(C-E-P)by encapsulating a fluorescent probe(P3)that combines the first-generation Grubbs catalyst with 6-methoxy-2-naphthylene in a hydrophobic pocket.The synthesis of probe P3 was confirmed through various structural characterization methods.Performance tests demonstrated that P3 exhibited a strong linear relationship with ethylene,rapid reaction time,and high selectivity.The C-E-P,created by encapsulating P3 with casein,facilitated fluorescence imaging of ethylene release from apple,bitter water rose,and aloe tissues.This approach allowed for observing changes in ethylene release between different plant tissues over time.Additionally,it was employed to monitor ethylene variations in the tissues of white ripe tomatoes during ripening,revealing that the peak ethylene release from the seeds occurs before that from the pericarp.The C-E-P developed in this study can effectively capture ethylene release information in plant tissues,providing an effective tool for fruit ripening and plant health monitoring in agricultural production.

关 键 词：植物组织荧光成像 乙烯 荧光探针 酪蛋白 

分 类 号：O657.3[理学—分析化学] O644[理学—化学]