不同抗性青稞品种响应啶磺草胺胁迫的代谢组差异分析  

Analysis of differences in metabolism of pyrimoxsulam resistance in different resistant barley varieties

作　　者：秦云卓 翁华[2] QIN Yunzhuo;WENG Hua(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Academy of Agricultural and Forestry Sciences,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海大学农林科学院,青海西宁810016

出　　处：《江西农业大学学报》2025年第2期314-324,共11页Acta Agriculturae Universitatis Jiangxiensis

基　　金：青海省重点研发与转化项目(2023-NK-154)。

摘　　要：【目的】挖掘青稞对啶磺草胺代谢过程中可能涉及的重要代谢物,明确青稞对啶磺草胺的代谢机制,为啶磺草胺的科学使用和耐除草剂的青稞品种选育提供理论依据。【方法】以啶磺草胺处理0,1,6d的耐药青稞品种(青0306)及敏感品种(青0160)的叶片为材料,采用液相色谱-质谱联用技术(LC-MS)进行分析。【结果】以VIP>1.0且P<0.05为阈值,筛选出598个差异代谢物,主要为黄酮化合物、萜类化合物重复、醇类化合物、苯酞类化合物等物质;KEGG代谢通路富集分析,主要参与代谢产物2-氧代羧酸代谢、氨基酸的生物合成以及类黄酮生物合成,以类黄酮生物合成最为显著。【结论】利用代谢组学技术,对不同品种青稞叶片在对啶磺草胺胁迫后的不同时期(0,1,6d)进行代谢组学分析,明确啶磺草胺处理后的敏感和耐药青稞品种的代谢物变化,并为后续的青稞遗传改良和农药科学施用提供依据。[Objective]This study aims to explore the important metabolites that may be involved in the metabolism of pyrimoxsulam in hulless barley,as well as clarify the metabolism mechanism of pyrimoxsulam in hulless barley,thus providing a theoretical basis for the scientific use of pyrimoxsulam and the selection and breeding of herbicide-tolerant hulless barley varieties.[Method]The leaves of a tolerant hulless barley variety(Qing 0306)and a sensitive variety(Qing 0160)treated with pyrimoxsulam for 0 d,1 d and 6 d were analyzed by liquid chromatography-mass spectrometry(LC-MS).[Result]With VIP>1.0 and P<0.05 as the threshold,598 differential metabolites were screened out,which were mainly flavonoids,terpenoids compounds,alcohols,phthalides and other substances;KEGG metabolic pathway was enriched and analyzed,which was mainly involved in metabolism of metabolites 2-oxocarboxylic acid metabolism,amino acid biosynthesis as well as flavonoid biosynthesis,among which the biosynthesis of flavonoids is the most significant.[Conclusion]Using metabolomics technology,different varieties of hulless barley leaves were analyzed by metabolomics in different periods(0,1,6 d)after pyrimoxsulam stress,the metabolite changes in sensitive and resistant hulless barley varieties after pyrimoxsulam treatment were clarified.This study provides a basis for the subsequent genetic improvement of hulless barley and the scientific application of pesticides.

关 键 词：青稞 啶磺草胺 代谢组学 差异代谢物 胁迫 耐药机制 

分 类 号：S512.3[农业科学—作物学]