葛根与植物乳植杆菌Ali.Plateau.Lp.Ⅷ联用对小鼠急性酒精性肝损伤的干预作用  

Protective Effect of Pueraria thomsonii Benth Combined with Lactiplantibacillus plantarum Ali.Plateau.Lp.ⅧAgainst Acute Alcoholic Liver Injury in Mice

作　　者：金国策 郑泽江 吴机伶 孙莲 左一帆 李啸 谈亚丽 黄蓉 贺海波 邹坤[1,2] JIN Guoce;ZHENG Zejiang;WU Jiling;SUN Lian;ZUO Yifan;LI Xiao;TAN Yali;HUANG Rong;HE Haibo;ZOU Kun(College of Biological&Pharmaceutical Sciences,China Three Gorges University,Yichang 443002,China;Hubei Key Laboratory of Natural Products Research and Development,China Three Gorges University,Yichang 443002,China;College of Medicine and Health Sciences,China Three Gorges University,Yichang 443002,China;Angel Biotechnology Co.,Ltd.,Yichang 443003,China)

机构地区：[1]三峡大学生物与制药学院,湖北宜昌443002 [2]三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌443002 [3]三峡大学健康医学院,湖北宜昌443002 [4]安琪生物科技有限公司,湖北宜昌443003

出　　处：《食品与生物技术学报》2025年第1期32-42,共11页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金青年基金项目(82204237);湖北省自然科学基金项目(2022CFB827)。

摘　　要：【目的】为了探究葛根(Pueraria thomsonii Benth)与植物乳植杆菌(Lactiplantibacillus plantarum)Ali.Plateau.Lp.Ⅷ(APLp.Ⅷ)联用对小鼠急性酒精性肝损伤的影响。【方法】建立小鼠急性酒精性肝损伤模型,将植物乳植杆菌APLp.Ⅷ与葛根联用,对比两者联用和单独使用对小鼠急性酒精性肝损伤的治疗效果。【结果】联用组可显著延长小鼠的攀附时间、爬杆时间与旷场中心活动距离,增强小鼠运动协调性和空间探索能力;联用组小鼠血液中乙醇质量浓度显著降低,乙醛脱氢酶(ALDH)活性显著提高;同时,谷丙转氨酶(AST)活性、谷草转氨酶(ALT)活性、丙二醛(MDA)水平显著降低;超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、总抗氧化能力(T-AOC)显著提高;肝组织病理切片显示小鼠肝损伤程度显著改善。【结论】植物乳植杆菌APLp.Ⅷ与葛根联用有协同增效预防小鼠酒精性肝损伤的作用。[Objective]The aim of the study is to investigate the effect of Pueraria thomsonii Benth combined with Lactiplantibacillus plantarum Ali.Plateau.Lp.Ⅷ(APLp.Ⅷ)on acute alcoholic liver injury in mice.[Method]A mouse model of acute alcoholic liver injury was established and treated by APLp.Ⅷand/or Pueraria thomsonii Benth.The therapeutic effect on acute alcoholic liver injury was compared between the group with APLp.Ⅷor Pueraria thomsonii Benth and the combination group.[Result]The mice in the combination group showed prolonged clinging and climbing time and an increased distance in the center in the open field test,which indicated that the combination enhanced the motor coordination and spatial exploration abilities of mice.Additionally,the blood of mice in the combination group showed a significant reduction in ethanol mass concentration and a significant increase in the activity of aldehyde dehydrogenase(ALDH).Furthermore,the combination group had significantly weakened activities of alanine aminotransferase(AST)and aspartate aminotransferase(ALT),a declined level of malondiadehyde(MDA),increased activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and enhanced total antioxidant capacity(T-AOC).Histopathological sections of the liver tissue showed significant alleviation of the injury in the combination group.[Conclusion]APLp.Ⅷcombined with Pueraria thomsonii Benth has synergistic effects in preventing alcoholic liver injury in mice.

关 键 词：葛根 植物乳植杆菌 急性酒精性肝损伤 乙醇代谢 氧化应激 

分 类 号：R285.5[医药卫生—中药学]