富硒黑豆蛋白预防苯并[a]芘诱导肝损伤的机制  

Mechanism of Selenium-enriched Black Soybean Protein in Preventing Benzo[a]pyrene-induced Liver Injury

作　　者：何紫妍 王珠琳 骆莹 李建科[1] 袁莉[1] HE Ziyan;WANG Zhulin;LUO Ying;LI Jianke;YUAN Li(College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710119,China)

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710119

出　　处：《食品与生物技术学报》2025年第1期43-53,共11页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金面上项目(31972183);陕西省技术创新引导专项项目(2022QFY09-03);西安市科技计划项目(21XJZZ0008)。

摘　　要：【目的】探究天然富硒黑豆蛋白(SeBSP,含硒质量分数84.00 mg/kg)对食品热加工有害产物苯并[a]芘(BaP)诱导的BALB/c小鼠肝损伤的预防作用。【方法】以正常黑豆蛋白(BSP,含硒质量分数0 mg/kg)为对照,分别对SeBSP和BSP进行了蛋白质表征检测,并灌胃处理小鼠。灌胃30 d后,对小鼠肝脏组织进行病理学观察,检测血清肝功能指标、肝脏氧化指标、BaP代谢相关指标以及细胞焦亡炎症因子水平。【结果】SeBSP和BSP均可预防BaP诱发的小鼠肝脏损伤,改善脂质代谢,降低丙二醛(MDA)和过氧化氢(H2O2)水平,提高谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(T-SOD)水平。与BSP相比,SeBSP可通过高效降低BaP代谢相关酶AhR的酶活力,以及CYP1A1、CYP1B1和GST-P1的mRNA相对水平,更好地抑制最终致癌物BPDE-DNA的形成,从而减轻BaP的肝脏毒性。同时,SeBSP可以更显著地降低NLRP3、ASC、Caspase-1、GSDMD和炎症因子(TNF-α、IL-1β、IL-18、iNOS、COX-2)的mRNA相对水平,从而减弱BaP诱导的肝脏焦亡性损伤。【结论】该研究揭示了SeBSP可通过抑制BaP对肝脏造成的氧化损伤,调节BaP代谢相关酶和减弱细胞焦亡,有效预防BaP诱导的肝损伤,为控制BaP肝脏毒性和拓展硒资源的功能提供了依据。[Objective]To investigate the preventive effect of natural selenium-enriched black soybean protein(SeBSP,selenium mass fraction is 84.00 mg/kg)on the liver injury induced by benzo[a]pyrene(BaP),a harmful product of high-temperature processed food,in BALB/c mice.[Method]This study used normal black bean protein(BSP,selenium mass fraction is 0 mg/kg)as a control.Both SeBSP and BSP,were characterized and used to feed BaP exposed mice by gavage.After 30 d of gavage,the liver tissues of mice were observed histopathologically,and the levels of serum liver function indicators,liver oxidative indicators,BaP metabolismrelated indicators and pyroptosis-related inflammatory factors were detected.[Result]Both SeBSP and BSP prevented the liver injury caused by BaP,improved lipid metabolism,reduced the levels of MDA and H2O2,and increased the levels of GSH-Px and T-SOD in mice.SeBSP outperformed BSP in inhibiting the formation of the final carcinogen BPDE-DNA by effectively reducing the activity of BaP metabolism-related enzyme AhR and the relative mRNA levels of CYP1A1,CYP1B1 and GSTP1,thus attenuating the hepatotoxicity of BaP.Moreover,SeBSP significantly down-regulated the relative mRNA levels of NLRP3,ASC,Caspase-1,GSDMD and inflammatory factors(TNF-α,IL-1β,IL-18,iNOS and COX-2),thereby ameliorating the BaP-induced pyroptotic injury in the liver.[Conclusion]The results revealed that SeBSP could effectively prevent BaP-induced liver injury by inhibiting BaP-induced oxidative damage to the liver,regulating BaP metabolism-related enzymes and reducing pyroptosis.The findings provide a basis for controlling the hepatotoxicity of BaP and expanding the functions of selenium resources.

关 键 词：苯并[A]芘 富硒黑豆蛋白 肝损伤 代谢相关酶 细胞焦亡 

分 类 号：TS201.4[轻工技术与工程—食品科学]