超分子弹性蛋白的促细胞增殖及抗氧化能力研究  

Cell Proliferation-promoting Effect and Antioxidant Capacity of Supramolecular Elastin

作　　者：王兆石 王召君 董明盛[1] 吴俊俊 WANG Zhaoshi;WANG Zhaojun;DONG Mingsheng;WU Junjun(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;College of Biological Engineering,Jiangnan University,Wuxi 214122,China)

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《食品与生物技术学报》2025年第1期132-142,共11页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金面上项目(31972060);国家自然科学基金青年基金项目(31601448);江苏省自然科学基金前沿引领技术基础研究专项项目(BK20202002);江苏省博士后科研资助计划项目(2018K030B)。

摘　　要：【目的】为解决动物体内的弹性蛋白不可溶性以及重组类弹性蛋白聚集的问题,提升其在功能性食品中的应用潜力。【方法】将昆虫弹性蛋白(resilin 16,R16)、链球菌中免疫球蛋白B1结构域(GB1)、酿酒酵母中Ure2的前1~80个序列Ure2(1-80)以及促进细胞黏附的RGD细胞结合位点进行连接,构建可溶性超分子弹性蛋白。测定超分子弹性蛋白对C2C12细胞的细胞毒性及生物安全性,并与透明质酸(hyaluronic acid,HA)和猪皮胶原蛋白进行促细胞增殖和抗氧化能力比较。【结果】超分子弹性蛋白对细胞无毒性,皮下注射无明显炎症反应,生物安全性高;自组装的原纤维可促进细胞增殖,对4种自由基的清除能力和对H_(2)O_(2)诱导的L929细胞损伤的保护作用均显著高于HA和猪皮胶原蛋白。【结论】该研究获得的超分子弹性蛋白生物安全性高,可以促进细胞增殖并且具有抗氧化能力,为开发功能性食品提供了新材料。[Objective]The aim of this study is to address the insolubility of elastin derived from animals and the aggregation of recombinant elastin-like proteins,and to enhance the elastin potential application in functional foods.[Method]In view of the above problems,resilin 16(R16)derived from insects,immunoglobulin G domain B1(GB1)from Streptococcus,and the Ure2 sequence of aa 1~80 Ure2(1-80)from Saccharomyces cerevicae were linked with the RGD sequence(a binding site of cells that promote cell adhesion)to construct the soluble supramolecular elastin.The cytotoxicity of the supramolecular elastin to C2C12 cells as well as its biosafety was evaluated.Furthermore,the supramolecular elastin was compared with hyaluronic acid(HA)and pigskin collagen in terms of the cell proliferation-promoting effect and antioxidant capacity.[Result]The supramolecular elastin had no toxic effect on cells and did not induce obvious inflammation after subcutaneous injection,demonstrating high biosafety.The self-assembled fibril promoted cell proliferation,and it showcased stronger scavenging ability against four kinds of free radicals and protective effect against H_(2)O_(2)-induced damage of L929 cells than HA and pigskin collagen.[Conclusion]The obtained supramolecular elastin has high biosafety,cell proliferation-promoting effect,and antioxidant capacity,serving as a new material for the development of functional food.

关 键 词：超分子弹性蛋白 生物安全 细胞增殖 氧化损伤保护 

分 类 号：TS201.2[轻工技术与工程—食品科学]