发酵条件对土曲霉菌丝球形态和黄曲霉毒素B_(1)降解效果的影响  

Effects of Fermentation Conditions on Mycelial Pellet Morphology of Aspergillus terreus and Aflatoxin B_(1) Degradation Efficency

作　　者：张洪鑫[1,2] 程思忠 王佳兴 谢岩黎 李倩[1,2] 孙淑敏 杨玉辉 ZHANG Hongxin;CHENG Sizhong;WANG Jiaxing;XIE Yanli;LI Qian;SUN Shumin;YANG Yuhui(College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China;Henan Provincial Key Laboratory of Grain,Oil and Food Safety Inspection and Control,Zhengzhou 450001,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001 [2]河南省粮油食品安全检测与控制重点实验室,河南郑州450001

出　　处：《食品与生物技术学报》2025年第1期143-153,共11页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金联合基金重点项目(U24A20473);河南省重点研发专项项目(231111113300)。

摘　　要：【目的】探究不同发酵条件对土曲霉(Aspergillus terreus,A.terreus)AterHaut1菌丝球形态和黄曲霉毒素B_(1)(AFB_(1))降解效果的影响。【方法】通过单因素试验对不同菌悬液接种体积分数、发酵温度和摇床转速条件下的菌丝球形态和AFB_(1)降解率进行考察,并通过Box-Behnken试验设计结合响应面分析法对发酵条件进行优化。【结果】得到的最佳发酵条件为:转速145 r/min、接种体积分数0.17%、发酵温度35℃。在最优条件下,发酵上清液的AFB_(1)实际降解率达96.34%,菌丝球平均直径为1.35 mm,均匀分布在1.20~1.50 mm。【结论】通过优化土曲霉AterHaut1的发酵条件可有效提高AFB_(1)降解率。[Objective]To study the effects of different fermentation conditions on the mycelial pellet morphology of Aspergillus terreus(A.terreus)AterHaut1 and aflatoxin B_(1)(AFB_(1))degradation efficency.[Method]Single factor experiments were carried out to investigate the mycelial pellet morphology and AFB_(1) degradation rates under different inoculation volume fractions,fermentation temperatures,and shaker speeds.The fermentation conditions were optimized by Box-Behnken design combined with response surface methodology.[Result]The optimal fermentation conditions were obtained as follows:shaker speed of 145 r/min,inoculation volume fraction of 0.17%,and fermentation temperature of 35℃.The actual degradation rate of AFB_(1) by the fermentation supernatant obtained under the optimal fermentation conditions reached 96.34%.At this time,the average diameter of mycelial pellets was 1.35 mm,with even distribution between 1.20 mm and 1.50 mm.[Conclusion]Optimizing the fermentation conditions of A.terreus AterHaut1 can improve the degradation rate of AFB_(1).

关 键 词：土曲霉 AFB_(1)降解酶 发酵 菌丝球 

分 类 号：S476[农业科学—农业昆虫与害虫防治]