红花总黄酮的超声辅助天然低共熔溶剂提取工艺优化  

Optimization of ultrasound‑assisted natural deep eutectic solvent extraction process of total flavonoids from safflower

作　　者：依明·尕哈甫[1,2,3] 周朝曦 艾尼娃尔·艾克木 古丽巴哈尔·卡吾力 YIMING·Gahafu;ZHOU Zhaoxi;AINIWAER·Aikemu;GULIBAHAER·Kawuli(School of Pharmacy,Xinjiang Medical University,Urumqi 830017,China;School of Pharmacy,Xinjiang Hetian College,Hetian 848000,China;Engineering Research Center of Quality Control of Uyghur Medicinal Materials and Preparation,Hetian 848000,China;Xinjiang Hetian Traditional Chinese Medicine and Ethnic Medicine Engineering Technology Research Center,Hetian 848000,China;Xinjiang Key Laboratory of Hetian Characteristic Chinese Traditional Medicine Research,Hetian 848000,China;Xinjiang Key Laboratory of Natural Drug Active Components and Drug Release Technology,Urumqi 83017,China)

机构地区：[1]新疆医科大学药学院,乌鲁木齐830017 [2]新疆和田学院药学院,新疆和田848000 [3]新疆维吾尔自治区维吾尔药材及制剂质量控制工程研究中心,新疆和田848000 [4]新疆和田特色中医药研究重点实验室,新疆和田848000 [5]新疆和田中药民族医药工程技术研究中心,新疆和田848000 [6]新疆天然药物活性组分与释药技术重点实验室,乌鲁木齐830011

出　　处：《包装与食品机械》2025年第2期50-57,共8页Packaging and Food Machinery

基　　金：新疆维吾尔自治区自然科学基金项目(2022D01C197);新疆天然药物活性成分与释药技术重点实验室项目(XJDX1713)。

摘　　要：为提高红花总黄酮的提取率,在料液比、超声功率、低共熔溶剂含水率和摩尔比等单因素试验基础上,通过响应面法优化超声提取工艺,采用体外试验分析红花总黄酮的乙酰胆碱酯酶(AchE)抑制活性。结果表明,红花总黄酮的最佳提取工艺为料液比1∶25(g/mL)、超声时间30 min、超声温度60℃、超声功率300 W、低共熔溶剂含水率37%、摩尔比1∶2,红花总黄酮的提取效率达5.07%,其AchE抑制率为65.52%。研究为红花总黄酮的开发利用提供技术支持。To enhance the extraction efficiency of total flavonoids from safflower,this study optimized an ultrasound-assisted extraction process using response surface methodology based on single-factor tests investigating solid-liquid ratio,ultrasonic power,water content in natural deep eutectic solvent(NADES),and molar ratio.The acetylcholinesterase(AChE)inhibitory activity of the extracted flavonoids was further assessed through in vitro assays.Results demonstrated that the optimal conditions included a solid-liquid ratio of 1∶25(g/mL),ultrasonic duration of 30 min,temperature of 60℃,power of 300 W,NADES water content of 37%,and molar ratio of 1∶2.Under these parameters,the total flavonoid extraction efficiency reached 5.07%,with an AChE inhibition rate of 65.52%.This work provides technical insights for advancing safflower flavonoid utilization in functional applications.

关 键 词：红花 总黄酮 天然低共熔溶剂 提取工艺 胆碱酯酶抑制活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]