响应面法结合深度神经网络优化刺五加果多糖提取工艺  

Optimization of polysaccharide extraction from acanthopanax senticosus fruits using response surface methodology and deep neural network

作　　者：苏适[1] 董立强[1] 黎莉[1] 王双侠[1] 王喜庆 张金凤[1] SU Shi;Dong Liqiang;LI Li;WANG Shuangxia;WANG Xiqing;Zhang Jinfeng(Faculty of Food and Pharmaceutical Engineering,Suihua University,Suihua 152000,China)

机构地区：[1]绥化学院食品与制药工程学院,黑龙江绥化152000

出　　处：《包装与食品机械》2025年第2期66-74,共9页Packaging and Food Machinery

基　　金：黑龙江省自然科学基金项目(LH2023H055);黑龙江省本科高校基本科研业务费项目(YWF10236240125);绥化学院药食同源特色产品开发与应用创新团队项目(240107100305)。

摘　　要：为提高刺五加果多糖的提取率,提出基于Box-Behnken响应面法与深度神经网络模型(deep neural network,DNN)协同优化的微波辅助离子液体提取工艺。通过响应面设计,筛选微波功率、离子液体浓度、提取时间及料液比等参数,并构建二次回归模型;利用DNN模型解析多因素间非线性关系,优化工艺条件。结果表明,DNN模型得到的最优工艺条件为微波功率350 W、离子液体浓度0.6 mol/L、提取时间35 min、料液比1∶24(g/mL),多糖提取率为16.71%,高于响应面法优化的提取工艺结果。体外抗氧化试验显示,刺五加果多糖对羟基自由基、DPPH自由基和ABTS^(+)·自由基的半数抑制浓度(IC_(50))分别为2.36,2.05,2.47 mg/mL。研究为刺五加果在功能性食品及抗衰老保健品开发中的应用提供理论依据。To enhance the extraction efficiency of polysaccharides from acanthopanax senticosus fruits,this study proposes a microwave-assisted ionic liquid extraction process through a synergistic optimization strategy combining Box-Behnken response surface methodology(RSM)and a deep neural network(DNN).Key parameters microwave power,ionic liquid concentration,extraction time,and (solid-liquid ratio)were screened via RSM experimental design,followed by the establishment of a quadratic regression model.The DNN was subsequently employed to decipher nonlinear interactions among multiple variables and refine process conditions.Results demonstrated that the DNN-optimized conditions(microwave) power 350 W,ionic liquid concentration 0.6 mol/L,extraction time 35 min,solid-liquid ratio 1∶24(g/mL)achieved a polysaccharide yield of 16.71%,surpassing the RSM-optimized results.In vitro antioxidant assays revealed IC50 values of 2.36,2.05,and 2.47 mg/mL against hydroxyl radicals,DPPH radicals,and ABTS⁺·radicals,respectively.This work provides a theoretical foundation for developing functional foods and anti-aging nutraceuticals from acanthopanax senticosus fruits.

关 键 词：刺五加果 多糖 工艺优化 响应面法 深度神经网络 抗氧化活性 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]