5种二氯苯酚类物质对斑马鱼胚胎的毒性效应研究  

Toxic Effect of Five Kinds of Dichlorophenols on Zebrafish Embryos

作　　者：章跃龙 王紫怡 章君麒 刘汉伟 ZHANG Yue-long;WANG Zi-yi;ZHANG Jun-qi(Ningbo Joysun Product Testing Service Company,Ningbo,Zhejiang 315000)

机构地区：[1]宁波中盛产品检测有限公司,浙江宁波315000 [2]宁波海关技术中心,浙江宁波315000

出　　处：《安徽农业科学》2025年第8期76-78,82,共4页Journal of Anhui Agricultural Sciences

基　　金：宁波中盛产品检测有限公司资助项目“基于斑马鱼实验模型的化妆品抗皱功效评价技术研究及应用”(2022ZS001)。

摘　　要：为了评估3,4-二氯苯酚(3,4-DCP)、2,5-二氯苯酚(2,5-DCP)、3,5-二氯苯酚(3,5-DCP)、2,6-二氯苯酚(2,6-DCP)和2,3-二氯苯酚(2,3-DCP)对斑马鱼胚胎的毒性效应,采用半静态暴露方法研究二氯苯酚类物质对斑马鱼胚胎的急性毒性和慢性毒性。结果表明,3,4-DCP、2,5-DCP、3,5-DCP、2,6-DCP和2,3-DCP对斑马鱼胚胎急性毒性的96 h LC 50分别为0.31、0.36、0.47、0.53和0.71 mg/L。3,4-DCP、2,5-DCP、3,5-DCP、2,6-DCP和2,3-DCP对斑马鱼胚胎32 d慢性毒性的最高无可观察效应浓度(NOEC)分别为15、24、24、38和38μg/L。以上研究结果表明,二氯苯酚对斑马鱼胚胎和胚胎发育具有一定的毒性效应。In order to evaluate the toxic effects of 3,4-dichlorophenol(3,4-DCP),2,5-dichlorophenol(2,5-DCP),3,5-dichlorophenol(3,5-DCP),2,6-dichlorophenol(2,6-DCP),and 2,3-dichloro-phenol(2,3-DCP)on zebrafish embryos,the semi-static exposure method was used to study the acute and chronic toxicity of dichlorophenols on zebrafish embryos.The results showed that the acute toxicity(96 h LC 50)of 3,4-DCP,2,5-DCP,3,5-DCP,2,6-DCP,and 2,3-DCP on zebrafish embryos was 0.31,0.36,0.47,0.53 and 0.71 mg/L,respectively.The highest no observed effect concentration(NOEC)of 3,4-DCP,2,5-DCP,3,5-DCP,2,6-DCP,and 2,3-DCP on zebrafish embryos after 32 d of exposure was 15,24,24,38 and 38μg/L,respectively.The above research showed that dichlorophenols showed certain toxic effects on zebrafish embryos and embryonic development.

关 键 词：斑马鱼 胚胎 急性毒性 慢性毒性 

分 类 号：X174[环境科学与工程—环境科学]