精细微观多孔结构木葡聚糖膜的制备及其体外性能研究  

Preparation and in Vitro Properties of Fine Microporous Xyloglucan Membrane

作　　者：刘桂亭 王宝群 邹圣灿 林莎莎 LIU Guiting;WANG Baoqun;ZOU Shengcan;LIN Shasha(Qingdao Chenlan Marine Biological Engineering Co.,Ltd.,Qingdao 266104,China)

机构地区：[1]青岛琛蓝海洋生物工程有限公司,山东青岛266104

出　　处：《山东化工》2025年第7期60-65,77,共7页Shandong Chemical Industry

摘　　要：设计了一种具有精细微观多孔结构的木葡聚糖膜的制备方法。采用水/四氢呋喃/1,4-二氧六环三元混合溶剂将木葡聚糖溶解后进行冻干,然后采用戊二醛溶液交联,再次冻干后制得。通过调节三元混合溶剂体积比调整木葡聚糖膜精细微观结构的孔隙率,而改变戊二醛的交联条件如交联剂用量、交联pH值以及交联时间可以改善膜的力学性能。通过傅里叶变换红外光谱(FT-IR)对木葡聚糖原料及交联后木葡聚糖膜进行结构表征,证明交联反应的进行。扫描电镜(SEM)分析表明木葡聚糖膜具有精细的多孔微观结构。理化检测所得的木葡聚糖膜具有良好的力学性能和吸液能力以及较低的细菌内毒素水平。通过体外细胞毒性及皮内反应试验证明该材料具有良好的生物相容性,同时建立大鼠的盲肠-腹壁损伤模型证明材料防粘连的有效性。A preparation method of xyloglucan membrane with fine microporous structure was designed.Xyloglucan was dissolved in water/tetrahydrofuran/1,4-dioxane and lyophilized,then crosslinked with glutaraldehyde solution and lyophilized again.By adjusting the volume ratio of ternary mixed solvent to adjust the fine microstructure porosity of the xylglucan membrane,and changing the crosslinking conditions such as the amount of crosslinking agent,crosslinking pH value and crosslinking time can improve the mechanical properties of the membrane.Fourier transform infrared spectroscopy(FT-IR)was used to characterize the structure of the xylodextran material and the film after crosslinking,which proved the cross-linking reaction.Scanning electron microscopy(SEM)analysis showed that the xylglucan membrane had fine porous microstructure.The xyloglucan membrane obtained by physicochemical detection has good mechanical properties and liquid absorption capacity,and low bacterial endotoxin level.In vitro cytotoxicity and intradermal reaction tests proved that the material had good biocompatibility.At the same time,the cecum-abdominal wall injury model of rats was established to prove the effectiveness of the material in preventing adhesion.

关 键 词：木葡聚糖膜 戊二醛交联 冻干 生物相容性 防粘连 

分 类 号：TQ464.1[化学工程—制药化工] O636[理学—高分子化学]