提取剂对酶联免疫法检测芝士粉中黄曲霉毒素M_(1)的影响  

Effect of Extracts on Determination of Aflatoxin M_(1) in Cheese Powde by ELISA

作　　者：伍蓝 黄华 戴晓红 方娟 马后扬 王丹 吴建勋 WU Lan;HUANG Hua;DAI Xiaohong;FANG Juan;MA Houyang;WANG Dan;WU Jianxun(Mengniu Dairy Products Wuhan Co.,Ltd.,Wuhan Hubei 430040)

机构地区：[1]蒙牛乳制品武汉有限责任公司,湖北武汉430040

出　　处：《中国乳业》2025年第4期84-88,共5页China Dairy

摘　　要：[目的]本研究使用酶联免疫法(ELISA法)评估了一级水、无水甲醇、二氯甲烷3种不同提取剂在检测芝士粉中黄曲霉毒素M_(1)含量方面的效果。[方法]通过对比一级水、无水甲醇、二氯甲烷3种不同提取剂提取效果的相对标准偏差,比较平均回收率。[结果]用一级水提取时,相对标准偏差2.56%,平均回收率35.30%~51.12%;用无水甲醇提取时,相对标准偏差4.20%,平均回收率81.32%~90.18%;用二氯甲烷提取时,样品组织状态异常,氮流蒸发后残留物呈油状,无法进行后续检测。[结论]以无水甲醇作为提取剂时,ELISA法的提取准确性较高,更适用于食品检测领域。[Objective]Enzyme-linked immunoassay(ELISA)was used to evaluate the effects of three different extractants of primary water,anhydrous methanol and dichloromethane on the determination of aflatoxin M_(1)in cheese powder.[Method]The average recovery rate was compared by comparing the relative standard deviation of extraction effects of primary water,anhydrous methanol and dichloromethane.[Result]When extracted with primary water,the relative standard deviation was 2.56%and the average recovery was 35.30%~51.12%.When extracted with anhydrous methanol,the relative standard deviation was 4.20%,and the average recovery was 81.32%~90.18%.When extracted with dichloromethane,the tissue status of the sample was abnormal,and the residue after nitrogen evaporation was oily,which could not be further determinated.[Conclusion]When extracted with anhydrous methanol.ELISA method had higher extraction accuracy and is more suitable for food determination.

关 键 词：酶联免疫法 芝士粉 黄曲霉毒素M_(1) 提取剂 

分 类 号：TS207.3[轻工技术与工程—食品科学]