基于碳量子点的荧光/比色适配体传感器检测金黄色葡萄球菌  

Detection of Staphylococcus aureus by fluorescence/colorimetric aptamer sensor based on carbon quantum dots

作　　者：马月圆 李静 韩高寰 张冰 高文超 MA Yueyuan;LI Jing;HAN Gaohuan;ZHANG Bing;GAO Wenchao(College of Biomedical Engineering,Tai University of Technology,Taiyuan 030024,China)

机构地区：[1]太原理工大学生物医学工程学院,太原030024

出　　处：《分析试验室》2025年第4期579-585,共7页Chinese Journal of Analysis Laboratory

基　　金：山西省青年科学基金(201901D211102)资助。

摘　　要：以碳量子点(CQDs)为荧光探针,金纳米粒子(Au NPs)为荧光猝灭剂和比色指示剂,适配体(Apt)为识别分子,构建了基于CQDs的适配体传感器并实现了金黄色葡萄球菌(S.a)的定量检测。检测体系以荧光强度恢复值(ΔF)为荧光信号响应,以Au NPs团聚特征峰处可见光吸收值与分散特征峰处可见光吸收值的比值(A_(650)/A_(520))为比色信号响应。在最佳检测条件下,荧光检测的线性范围为2.620×10^(2)~2.620×10^(4) cfu/mL,检测限为0.422 cfu/mL;比色检测的线性范围为2.620×10^(4)~5.241×10^(6) cfu/mL,检测限为252 cfu/mL。所构建的检测体系可实现S.a的高灵敏、可视化检测。An aptamer sensor is constructedto achieve the quantitative detection of Staphylococcus aureus(S.a)by using carbon quantum dots(CQDs)as the fluorescence probe,Au nanoparticles(Au NPs)as the fluorescence quencher and colorimetric indicator,and aptamer(Apt)as the recognition molecule.The fluorescence intensity recovery value(ΔF)was the fluorescence signal response and the ratio of visible light absorption value of Au NPs in different states(A_(650)/A_(520))was the colorimetric signal response of the detection system.Under the optimal detection conditions,the linear range of fluorescence detection was 2.620×10^(2)-2.620×10^(4) cfu/mL with the detection limit of0.422 cfu/mL.Furthermore,the linear range of colorimetric detection was 2.620×10^(4)-5.241×10^(6) cfu/mL with the detection limit of 252 cfu/mL.The constructedsystem can achieve highly sensitive and visual detection of S.a.

关 键 词：碳量子点 内滤效应 适配体传感器 荧光/比色检测 金黄色葡萄球菌 

分 类 号：TS207.4[轻工技术与工程—食品科学] O652.1[轻工技术与工程—食品科学与工程]