α5-nAChR对慢性应激肺腺癌荷瘤小鼠疑核c-Fos表达的影响  

Effect ofα5-nAChR on the expression of c-Fos in nucleus ambiguus in chronic stress-induced lung adenocarcinoma mice

作　　者：蔡佳莹 王靖婷 王增萍 王璟 郏雁飞 马晓丽 CAI Jiaying;WANG Jingting;WANG Zengping;WANG Jing;JIA Yanfei;MA Xiaoli(Research Center of Basic Medicine,Central Hospital Affiliated to Shandong First Medical University,Jinan 250013,Shandong,China;Department of Medical Laboratory,Shandong Second Medical University,Weifang 261053,Shandong,China)

机构地区：[1]山东第一医科大学附属中心医院基础医学研究中心,山东济南250013 [2]山东第二医科大学医学检验学院,山东潍坊261053

出　　处：《山东大学学报(医学版)》2025年第4期69-74,共6页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金面上项目(ZR2021MH322)。

摘　　要：目的探讨α5-烟碱型乙酰胆碱受体(α5-nicotinic acetylcholine receptor,α5-nAChR)对慢性不可预见性应激(chronic unpredictable mild stress,CUMS)肺腺癌荷瘤小鼠疑核c-Fos表达的影响。方法运用免疫组织化学技术检测NC+CUMS组(n=6)、CUMS+CHRNA5^(+/+)组(n=6)及CUMS+CHRNA5^(+/+)+Mec组(n=6)肺腺癌荷瘤小鼠瘤组织中α5-nAChR与Ki67的表达及相关性;运用免疫荧光技术检测NC组(n=6)、NC+CUMS组(n=6)、CUMS+CHRNA5^(+/+)组(n=6)及CUMS+CHRNA5^(+/+)+Mec组(n=6)肺腺癌荷瘤小鼠疑核c-Fos的表达。结果α5-nAChR与Ki67的表达呈正相关(P<0.05),α5-nAChR介导慢性应激促进肺腺癌细胞增殖;α5-nAChR表达与疑核c-Fos的表达呈正相关(P<0.05)。结论α5-nAChR调控慢性应激肺腺癌荷瘤小鼠疑核c-Fos的表达。Objective To investigate the effect of α5-nicotinic acetylcholine receptor(α5-nAChR)on the expression of c-Fos in nucleus ambiguus in chronic unpredictable mild stress(CUMS)-induced lung adenocarcinoma mice.Methods The expression and correlation between α5-nAChR and Ki67 in tumour tissues of the NC+CUMS group(n=6),the CUMS+CHRNA5+/+group(n=6)and the CUMS+CHRNA5^(+/+)+Mec group(n=6)were examined using immunohistochemistry in lung adenocarcinoma mice.Immunofluorescence technique was used to detect the expression of c-Fos in nucleus ambiguus of the NC group(n=6),the NC+CUMS group(n=6),the CUMS+CHRNA5^(+/+)group and the CUMS+CHRNA5^(+/+)+Mec group(n=6)in lung adenocarcinoma mice.Resultsα5-nAChR was positively correlated with the expression of Ki67(P<0.05)andα5-nAChR mediates chronic stress-induced lung adenocarcinoma cell proliferation.α5-nAChR expression was positively correlated with the expression of c-Fos in nucleus ambiguus(P<0.05).Conclusion α5-nAChR mediates the expression of c-Fos in nucleus ambiguus in chronic stress-induced lung adenocarcinoma mice.

关 键 词：α5-烟碱型乙酰胆碱受体 C-FOS 疑核 慢性应激 肺腺癌 小鼠 

分 类 号：R734.2[医药卫生—肿瘤]