调控超甜玉米种子贮藏物质利用率GWAS分析及候选基因挖掘  

GWAS Analysis and Identification of Candidate Genes for Regulating the Utilization of Storage Material in Super Sweet Corn Seeds

作　　者：余育林 刘杰 王东兴 孙涛 崔妮妮 陈磊 程昕昕 余海兵 YU Yu-lin;LIU Jie;WANG Dong-xing;SUN Tao;CUI Ni-ni;CHEN Lei;CHENG Xin-xin;YU Hai-bing(College of Agriculture,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院农学院,安徽凤阳233100

出　　处：《玉米科学》2025年第4期34-41,共8页Journal of Maize Sciences

基　　金：安徽省高校自然科学研究重大项目(KJ2019ZD57);安徽科技学院科研发展基金项目(FZ230126)。

摘　　要：利用玉米56K基因芯片获得的SNP标记,采用MLM(Q+K)模型,对种子贮藏物质利用率进行全基因组关联分析(GWAS)及候选基因挖掘,揭示超甜玉米种子贮藏物质利用率的遗传特性。结果表明,共定位到6个调控种子贮藏物质利用率显著关联的SNP位点,贡献率范围在14.5%~23.8%、表型变异解释率在1.1%~20.0%,其中2个SNP位点距前人定位关联SNP位点0.7 Mb的范围内。在显著SNP位点上下游200 kb的置信区间内,共检测到184个候选基因,其中已注释基因62个,占比为33.7%。结合文献资料分析筛选出18个候选基因调控种子贮藏物质利用率,主要参与激素合成与信号转导、细胞分裂、蛋白质和酶合成等过程,为深入理解超甜玉米种子贮藏物质利用率的遗传机制提供线索,有助于进一步研究种子贮藏物质利用率相关性状基因的定位和克隆。To elucidate the genetic characteristics of seed storage substance utilization in super sweet corn,this study utilized SNP markers obtained from the maize 56K gene chip and employed the MLM(Q+K)model to conduct genome-wide association studies(GWAS)and identify candidate genes.The results showed that total of 6 SNPs loci significantly associated with regulating seed storage material utilization were detected,with the contribution rates ranged from 14.5%to 23.8%and phenotypic variance explained rates ranged from 1.1%to 20.0%.The explained phenotypic variance ranged from 1.1%to 20.0%,with two SNP loci within 0.7 Mb of the previous locus-associated SNP locus.A total of 184 genes were detected within the 200 kb confidence interval upstream and downstream of the significant SNPs loci,among which 62 genes were annotated,accounting for 33.7%.Based on literature analysis,18 genes were screened out to regulate the utilization rate of seed storage material,which are mainly involved in the pathways of hormone synthesis and signal transduction,cell division,protein and enzyme synthesis,providing impor⁃tant indication for the further study of genetic mechanism of seed storage material utilization in super sweet corn.This provides important indication for understanding the genetic mechanism of seed storage material utilization,and helps further research on the location and cloning of seed storage material utilization-related trait genes.

关 键 词：超甜玉米 种子贮藏物质利用率 全基因组关联分析 候选基因 

分 类 号：S513.035.3[农业科学—作物学]