CLDN9 Levels Influence the Biological Activities of Cells in Gastric Cancer  

作　　者：Jin Liu Xiu Zhang Xiao Hu Gaofeng Yuan Kai Chen 

机构地区：[1]Department of Oncology,The First Affiliated Hospital of Soochow University,Suzhou,215000,China [2]Department of Oncology,Suqian First Hospital,Suqian,223800,China

出　　处：《BIOCELL》2025年第4期664-679,共16页生物细胞(英文)

基　　金：supported by the Science and Technology Plan of Suqian City(Grant No.S202117).

摘　　要：Objective:This study examines the significance and functions of CLDN9 in gastric cancer(GC),intending to identify novel targets for diagnosis and treatment.Methods:CLDN9 expression in GC tissues and cell lines was investigated in TCGA data,with analysis with Western blotting,qRT-PCR,and immunohistochemical analyses.Correlations between clinicopathological characteristics,progression-free survival(PFS),and overall survival(OS)were assessed with Cox regression.The effect of CLDN9 knockdown/overexpression on tumorigenic functions(proliferation,migration,and invasion)was assessed using CCK-8,colony formation,and Transwell assays.Tumor-bearing assays were performed to verify the impact of CLDN9 knockdown on the in vivo proliferation of GC cells.Results:Analysis of TCGA data,qRT-PCR,Western blotting,and immunohistochemistry indicated significantly elevated levels of CLDN9 in GC tissues and cell lines(p 0.01).Compared to the group with high CLDN9 expression,<PFS and OS were markedly longer in cases with low CLDN9 levels(p 0.05).Multivariate regression indicated that<CLDN9 levels were independently predictive of GC prognosis.CCK-8,Transwell,and colony formation tests showed that CLDN9 overexpression promoted GC cell proliferation,clonogenicity,and migratory/invasive capabilities,but CLDN9 knockdown markedly reduced these features.Experiments involving tumor-bearing models demonstrated that the suppression of CLDN9 inhibited the in vivo growth of GC cells.Conclusion:In conclusion,this study’s findings suggested that CLDN9 might be a valuable therapeutic target or diagnostic biomarker for GC.

关 键 词：Gastric cancer CLDN9 cell proliferation INVASION MIGRATION 

分 类 号：R735.2[医药卫生—肿瘤]