角膜疾病相关生物标志物的代谢组学研究  

Application of metabolomics in the analysis of biomarkers associated with corneal diseases

作　　者：蓝诚红[1] 王烽 Lan Chenghong;Wang Feng(Department of Ophthalmology,Meizhou People’s Hospital,Meizhou Guangdong 514000,China)

机构地区：[1]梅州市人民医院眼科,广东梅州514000

出　　处：《国际眼科纵览》2025年第2期141-146,共6页International Review of Ophthalmology

基　　金：广东省梅州市医药卫生科研立项课题(2023-B-32);广东省梅州市人民医院科研培育项目(PY-C2021060)。

摘　　要：早期识别诊断生物标志物对降低角膜疾病相关视力障碍的风险至关重要。代谢组学是对全基因组或蛋白质组相互作用产生的生物体液或组织中代谢物的研究。目前质谱法和核磁共振波谱已应用于角膜、泪液和房水分析中以识别角膜疾病相关的生物标志物。感染性角膜炎相关代谢物如RVD3、18-HEPE、11(12)-EET等系与感染致病期相关的介质。炎症因子(如花生四烯酸和亚油酸)以及短链有机酸(如柠檬酸、丙酮酸、琥珀酸、草酰乙酸和谷氨酸)的上调与配戴软性隐形眼镜后眼不适及其相关疾病的代谢机制有关。角膜屈光手术后相关代谢物如抗坏血酸、牛磺酸、亚精胺、组氨酸和花生四烯酸等主要与炎症、氧化、神经保护和再生有关。前列腺素F2α、前列腺素A2、16,16-二甲基前列腺素E2和5-羟基二十碳四烯酸等代谢物上调与圆锥角膜的发病有关。III型胶原蛋白、鞘氨醇、肌醇等代谢物上调可能是糖尿病性角膜病变的生物标志物。Early identification of diagnostic biomarkers is essential to reduce the risk of cornea-related visual impairment.Metabolomics is the study of metabolites in the fluids or tissues of organisms resulting from genome-wide or proteomic interactions.Mass spectrometry and nuclear magnetic resonance are now used in corneal,tear and atrial fluid analysis to identify biomarkers associated with corneal diseases.Infectious keratitis-associated metabolites,such as RVD3,18-HEPE,and 11(12)-EET,reveal mediators associated with the pathogenic phase of the infection.Inflammatory factors(e.g.,arachidonic acid and linoleic acid),and upregulation of short-chain organic acids(e.g.,citric acid,pyruvic acid,succinic acid,oxaloacetic acid and glutamate),revealed metabolic mechanisms of ocular discomfort and related disorders after soft contact lens wear.Post-corneal refractive surgery-related metabolites such as ascorbic acid,taurine,spermidine,histidine and arachidonic acid,suggested metabolites primarily associated with inflammation,oxidation,neuroprotection,and regeneration.Upregulated prostaglandin F2α,Prostaglandin A2,16,16-dimethylprostaglandin E2,and 5-hydroxy eicosatetraenoic acid could help to identify patients with at-risk cone corneas.Upregulation of metabolites such as collagen subtype III,sphingomyelin,and inositol had the potential to be used as a biomarker for diabetic keratopathy.

关 键 词：角膜疾病 代谢组学 

分 类 号：R772.2[医药卫生—眼科]