原儿茶酸通过抑制IL-6/JAK1/STAT5信号通路抑制颅内动脉瘤进展  

Protocatechuic acid inhibits the progression of intracranial aneurysms by inhibiting the IL-6/JAK1/STAT5 signaling pathway

作　　者：闵敬亮 郭亚玲 张辉 王大巍 MIN Jingliang;GUO Yaling;ZHANG Hui;WANG Dawei(Department of Neurosurgery,the Second Affiliated Hospital of Bengbu Medical University,Bengbu 233000,China;The First Affiliated Hospital of Bengbu Medical University)

机构地区：[1]蚌埠医科大学第二附属医院神经外科,安徽蚌埠233000 [2]蚌埠医科大学第一附属医院

出　　处：《包头医学院学报》2025年第4期34-38,共5页Journal of Baotou Medical College

基　　金：2023年度蚌埠医科大学自然科学重点项目(2023byzd102)。

摘　　要：目的:探讨原儿茶酸通过抑制IL-6/JAK1/STAT5信号通路抑制颅内动脉瘤进展的分子机制。方法:构建颅内动脉瘤大鼠模型,分组为NC组(空白对照组)、颅内动脉瘤组(CA组)、颅内动脉瘤模型+原儿茶酸组(CA+PCA组),影像学检测各组颅内血管壁内径,HE染色检测各组血管内皮情况。ELISA检测各组外周血中IL-6、JAK1和STAT5的表达,RT-PCR和WB检测各组组织样本中IL-6、JAK1和STAT5的表达水平。结果:相较于NC组,从第8周开始,CA组的血管壁厚度明显增高(P<0.05),而CA+PCA组的血管壁厚度较CA组降低。HE染色结果显示,相较于NC组,CA组的血管内皮中巨噬细胞增多,而CA+PCA较CA组血管内皮巨噬细胞减少。ELISA实验显示,相较于NC组,CA组的IL-6、JAK1和STAT5表达升高,而CA+PCA组中IL-6、JAK1和STAT5表达较CA组降低。RT-PCR和WB结果与ELISA结果相同(P<0.05)。结论:原儿茶酸通过抑制IL-6/JAK1/STAT5信号通路可抑制颅内动脉瘤进展。Objective:To investigate the molecular mechanism of protocatechuic acid inhibiting the progression of intracranial aneurysms by inhibiting IL-6/JAK1/STAT5 signaling pathway.Methods:The rat model of intracranial aneurysm was constructed and divided into NC group(blank control group),intracranial aneurysm group(CA group)and intracranial aneurysm model+protocatechuic acid group(CA+PCA group).The inner diameter of intracranial vascular wall in each group was detected by imaging,and the vascular endothelium in each group was detected by HE staining.The expression of IL-6,JAK1 and STAT5 in peripheral blood of each group was detected by ELISA,and the expression levels of IL-6,JAK1 and STAT5 in tissue samples of each group were detected by RT-PCR and WB.Results:Compared with the NC group,the thickness of the vascular wall in the CA group was significantly increased from the eighth week(P<0.05),while the thickness of the vascular wall in the CA+PCA group was lower than that in the CA group.The results of HE staining showed that compared with the NC group,the number of macrophages in the vascular endothelium of the CA group increased,while the number of macrophages in the vascular endothelium of the CA+PCA group decreased compared with the CA group.ELISA experiments showed that the expression of IL-6,JAK1 and STAT5 in CA group was higher than that in NC group,while the expression of IL-6,JAK1 and STAT5 in CA+PCA group was lower than that in CA group.RT-PCR and WB results were the same as ELISA results(P<0.05).Conclusion:Protocatechuic acid inhibits the progression of intracranial aneurysms by inhibiting the IL-6/JAK1/STAT5 signaling pathway.

关 键 词：原儿茶酸 IL-6 JAK1/STAT5信号通路 颅内动脉瘤模型 

分 类 号：R743[医药卫生—神经病学与精神病学]