基于网络药理学探讨丹皮酚治疗宫颈癌的作用机制  

Exploring the mechanism of action of paeonol in the treatment of cervical cancer based on network pharmacology

作　　者：李克明 刘亚鲁[3] 隆毅[4] 杨淑清 高星 丁海玲 王雪卉 李艺辉 冯程 LI Keming;LIU Yalu;LONG Yi;YANG Shuqing;GAO Xing;DING Hailing;WANG Xuehui;LI Yihui;FENG Cheng(School of Medicine,Qilu Institute of Technology,Jinan 250200,China;School of Medicine,Shandong Xiehe University,Jinan 250100,China;Department of Pharmacy,Jinan Children's Hospital,Jinan 250022,China;Department of Pharmacy,the Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250000,China;Jinan Municipal People's Government Organs Outpatient Department,Jinan 250000,China)

机构地区：[1]齐鲁理工学院医学院,山东济南250200 [2]山东协和学院医学院,山东济南250100 [3]济南市儿童医院医学部,山东济南250022 [4]山东中医药大学第二附属医院药学部,山东济南250000 [5]济南市人民政府机关门诊部,山东济南

出　　处：《老年医学研究》2025年第2期37-40,共4页Geriatrics Research

基　　金：山东省中医药科技项目(Z-2023055,牡丹叶通过PI3K/AKT信号通路抗宫颈癌作用机制研究)。

摘　　要：目的探讨丹皮酚治疗宫颈癌的作用机制。方法通过TCMSP、PubChem、STITCH和BATMAN-TCM数据库筛选丹皮酚相关靶点,结合Gene Cards和OMIM数据库筛选宫颈癌相关靶点,筛选出交集靶点;经STRING构建蛋白质—蛋白质相互作用(PPI)网络及Cytoscape拓扑分析筛选核心靶点;利用仙桃学术平台进行基因本体(GO)功能富集分析与京都基因和基因组百科全书(KEGG)通路富集分析。结果筛选得到丹皮酚作用靶点109个,宫颈癌作用靶点1372个,丹皮酚治疗宫颈癌潜在靶点28个。PPI筛选出苏氨酸激酶1(AKT1)、肿瘤坏死因子(TNF)、半胱天冬蛋白酶-3(CASP3)和B淋巴细胞瘤-2基因(BCL2)为丹皮酚治疗宫颈癌关键靶点。GO功能富集分析显示,丹皮酚主要靠转录共激活因子结合、外膜、外源性凋亡信号通路等过程发挥作用;KEGG通路富集分析涉及PI3K-AKT及NF-κB信号通路。结论丹皮酚可能通过靶向调控AKT1、TNF、CASP3和BCL2介导的PI3K-AKT及NF-κB信号通路治疗宫颈癌。Objective To analyze the mechanism of action of paeonol in cervical cancer treatment.Methods Pae-onol-related targets were screened through databases including TCMSP,PubChem,STITCH and BATMAN-TCM.Cervical cancer-related targets were identified using Gene Cards and OMIM databases,followed by screening for intersection tar-gets.A protein-protein interaction(PPI)network was constructed via STRING,and core targets were selected through to-pological analysis using Cytoscape.Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using Xiantao Academic.Results A total of 109 paeonol-related targets and 1372 cervical cancer-related targets were screened,with 28 potential anti-cervical cancer tar-gets obtained.PPI analysis identified core targets including AKT1,TNF,CASP3,and BCL2.GO functional enrichment analysis showed that paeonol primarily acts through processes such as transcription coactivator binding,outer membrane,and extrinsic apoptotic signaling pathway.KEGG pathway enrichment analysis involved the PI3K-AKT and NF-κB signal-ing pathways.Conclusion Paeonol may treat cervical cancercervical cancer by targeting and regulating AKT1,TNF,CASP3 and BCL2 to mediate the PI3K-AKT and NF-κB signaling pathways.

关 键 词：丹皮酚 宫颈癌 网络药理学 作用机制 

分 类 号：R737.33[医药卫生—肿瘤]