细菌性脑膜炎患儿血清中miR-17-5p和miR-141-3p水平及临床意义  

Levels and clinical significance of miR-17-5p and miR-141-3p in the serum of children with bacterial meningitis

作　　者：谭自明 张美[1] 李彤 王君[1] 罗琼[1] TAN Ziming;ZHANG Mei;LI Tong;WANG Jun;LUO Qiong(Department of Pediatric Neurology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830000,China;College of Pediatrics,Xinjiang Medical University,Urumqi,Xinjiang 830000,China)

机构地区：[1]新疆医科大学第一附属医院儿童神经内科,新疆乌鲁木齐830000 [2]新疆医科大学儿科学院,新疆乌鲁木齐830000

出　　处：《国际检验医学杂志》2025年第9期1051-1055,共5页International Journal of Laboratory Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C313)。

摘　　要：目的探究细菌性脑膜炎(BM)患儿血清中微小RNA(mi R)-17-5p、mi R-141-3p水平及临床意义。方法选取新疆医科大学第一附属医院2019年5月至2022年5月收治的111例BM患儿作为研究组,另选取同期111例健康体检儿童作为对照组。采用实时荧光定量PCR(q RT-PCR)检测血清mi R-17-5p、mi R-141-3p的水平;采用Pearson相关性分析BM患儿血清mi R-17-5p、mi R-141-3p水平与炎症因子的相关性,采用多因素Logistic回归分析BM发生的影响因素,采用受试者工作特征(ROC)曲线分析mi R-17-5p、mi R-141-3p水平对BM的诊断价值。结果研究组血清mi R-17-5p、mi R-141-3p水平比对照组显著降低(P<0.05),研究组血清中C反应蛋白(CRP)、降钙素原(PCT)、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6水平比对照组显著升高(P<0.05);Pearson相关性分析显示,mi R-17-5p、mi R-141-3p与CRP、PCT、IFN-γ、TNF-α、IL-1β、IL-6呈负相关(P<0.05);多因素Logistic分析结果显示,CRP、PCT、IFN-γ、TNF-α、IL-1β、IL-6是影响BM发生的危险因素(P<0.05),mi R-17-5p、mi R-141-3p是影响BM发生的保护因素(P<0.05);根据ROC曲线分析得知,血清mi R-17-5p水平诊断BM的曲线下面积(AUC)为0.756,血清mi R-141-3p水平诊断BM的AUC为0.720,二者联合诊断BM的AUC为0.819,二者联合的AUC较单项检测更大(Z联合vs.mi R-17-5p=2.278、Z联合vs.mi R-141-3p=2.425,P<0.05)。结论BM患儿血清中mi R-17-5p、mi R-141-3p水平降低,二者与炎症因子水平有关,且二者联合检测对BM诊断价值较高。Objective To investigate the levels and clinical significance of micro RNA(mi R)-17-5p and mi R-141-3p in the serum of children with bacterial meningitis(BM).Methods A total of 111 children with BM admitted to the First Affiliated Hospital of Xinjiang Medical University from May 2019 to May 2022 were included as the study group,and another 111 healthy children who underwent physical examinations were included as the control group.Real-time fluorescence quantitative PCR(q RT-PCR)was used to measure the expression levels of serum mi R-17-5p and mi R-141-3p.Pearson correlation was used to analyze the correlation between serum mi R-17-5p,mi R-141-3p levels and inflammatory factors in children with BM.Multivariate Logistic regression was applied to analyze the influencing factors of BM occurrence.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of mi R-17-5p and mi R-141-3p levels for BM.Results The serum levels of mi R-17-5p and mi R-141-3p in the study group were obviously lower than those in the control group(P<0.05),while the serum levels of C-reactive protein(CRP),procalcitonin(PCT),interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,and IL-6 in the study group were higher than those in the control group(P<0.05).According to Pearson correlation analysis,mi R-17-5p and mi R-141-3p were negatively correlated with CRP,PCT,IFN-γ,TNF-α,IL-1β,and IL-6(P<0.05).According to multivariate Logistic analysis,CRP,PCT,IFN-γ,TNF-α,IL-1β,and IL-6 were risk factors affecting the occurrence of BM(P<0.05),while mi R-17-5p and mi R-141-3p were protective factors affecting the occurrence of BM(P<0.05).According to the ROC curve,the area under the curve(AUC)of serum level of mi R-17-5p for diagnosing BM was 0.756,and the AUC of serum level of mi R-141-3p for diagnosing BM was 0.720.The AUC of the combination of the two for diagnosing BM was 0.819,which was larger than that of single detection(Zcombination vs.mi R-17-5p=2.278,Zcombination vs.mi R-141-3p=2.425,P<0.05).C

关 键 词：细菌性脑膜炎 微小RNA-17-5p 微小RNA-141-3p 诊断 

分 类 号：R742.9[医药卫生—神经病学与精神病学]