乳铁蛋白检测方法研究进展  

Research advances on detection of lactoferrin

作　　者：叶颖慧 徐明轩 梁敏慧 张冬梅 张晓敏 刘忠伟 王思程 YE Yinghui;XU Mingxuan;LIANG Minhui;ZHANG Dongmei;ZHANG Xiaomin;LIU Zhongwei;WANG Sicheng(Chinese Academy of Inspection and Quarantine,Beijing,100123,China)

机构地区：[1]中国检验检疫科学研究院,北京100123

出　　处：《质量安全与检验检测》2025年第2期49-53,57,共6页QUALITY SAFETY INSPECTION AND TESTING

基　　金：国家市场监督管理总局科技计划项目(2022MK198)。

摘　　要：乳铁蛋白是一种重要的非血红素铁结合糖蛋白,具有抗菌、抗炎、促进铁吸收和调节免疫等生理作用,乳铁蛋白的含量测定在营养学、食品科学领域尤为重要。本文针对目前不同样品中乳铁蛋白含量的检测方法进行了阐述,主要包括高效液相色谱法(HPLC)、高效液相色谱-串联质谱法(HPLC-MS)、酶联免疫法(ELISA)、电泳法、免疫传感器法等,深入探讨各种乳铁蛋白检测方法的原理、特点和应用,对比不同方法的优缺点。Lactoferrin is an important non-heme iron-binding glycoprotein,with physiological functions such as antibacterial,anti-inflammatory,promoting iron absorption and immune regulation.The determination of lactoferrin content is particularly significant in the fields of nutrition and food science.This paper elaborates on the current methods for detecting lactoferrin content in different samples,including high-performance liquid chromatography(HPLC),high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS),enzyme-linked immunosorbent assay(ELISA),electrophoresis,and immunosensor methods.The paper delves into the principles,characteristics and applications of various lactoferrin detection methods,comparing the advantages and disadvantages of different approaches.

关 键 词：乳铁蛋白 检测方法 研究进展 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]