多方法鉴定食品中单核细胞增生李斯特氏菌  

Multiple Methods Identification of Listeria monocytogenes in Food

作　　者：邱莎 曾博 QIU Sha;ZENG Bo(Deyang Food and Drug Safety Inspection and Inspection Center,Deyang,Sichuan 618000,China)

机构地区：[1]德阳市食品药品安全检验检测中心,四川德阳618000

出　　处：《农产品加工》2025年第8期86-91,共6页Farm Products Processing

摘　　要：通过参加食品中单核细胞增生李斯特氏菌能力验证计划、丰富检验思路,探索食品中单核细胞增生李斯特氏菌的快速检测及鉴定。按照食品中单核细胞增生李斯特氏菌能力验证作业指导书和《食品安全国家标准食品微生物学检验单核细胞增生李斯特氏菌检验》(GB 4789.30—2016),通过常规生化鉴定、VITEK2全自动微生物鉴定系统、琼脂糖凝胶电泳和16S rRNA基因序列4种方法对TF04330007和TF04330008这2个样品进行单核细胞增生李斯特氏菌的检验。结果表明,编号TF04330007样品检出单核细胞增生李斯特氏菌,编号TF04330007未检出单核细胞增生李斯特氏菌。实验室4种检验方法结果一致,能力验证结果满意。在常规生化鉴定的基础上,辅助VITEK2全自动微生物鉴定系统、琼脂糖凝胶电泳和16S rRNA基因序列进行检测,能够缩短检测时间,特异性和敏感性菌也较好,4种方法互相补充可确保试验结果的准确性。Through participating the Proficiency testing of Listeria monocytogenes qualitative in food,enrich inspection ideas to explore the rapid detection and identification of Listeria monocytogenes in food.Samples were diluted according to the Proficiency testing Guidance and National Food Safety Standard-Food Microbiology Inspection-Listeria monocytogenes(GB 4789.30—2016),two samples(TF04330007 and TF04330008)were tested for Listeria monocytogenes by routine biochemical identification,VITEK2 automatic microbial identification system,agarose gel electrophoresis and 16S rRNA gene sequence.The results showed that Listeria monocytogenes was detected in sample TF04330007,while no Listeria monocytogenes was detected in sample TF04330008.The results of the four methods were consistent,and the results were satisfactory.On the basis of routine biochemical identification,assisted VITEK2 automatic microbial identification system,agarose gel electrophoresis and 16S rRNA gene sequence could shorten the detection tine,with good specificity and sensitivity.

关 键 词：单核细胞增生李斯特氏菌 能力验证 16S rRNA基因序列 

分 类 号：O655[理学—分析化学]