间接免疫荧光法改进的效果评价  

Evaluation of Improved Indirect Immunofluorescence Method

作　　者：孙玉婷 史燕 刘燕坤 田锋 卢耀勤[2] 张建明 耿合员 马雪征 夏侯为民 SUN Yuting;SHI Yan;LIU Yankun;TIAN Feng;LU Yaoqin;ZHANG Jianming;GENG Heyuan;MA Xuezheng;XIAHOU Weimin(Edical Laboratory Department,Xinjiang International Travel Health Care Center(Urumqi Customs Port Clinic),Urumqi Xinjiang 830000,China;Office of Urumqi Center for Disease Control and Prevention,Urumqi Xinjiang 830023,China;Department of Clinical Laboratory,The First Hospital of Quanzhou,Quanzhou Fujian 362000,China;Jiangsu International Travel Health Care Center(Nanjing Customs Port Outpatient Department),Nanjing Jiangsu 210004,China;Institute of Health and Quarantine,Chinese Academy of Inspection and Quarantine,Beijing 100123,China;Laboratory of Comprehensive Technical Service Center,Shaoguan Customs,Shaoguan Guangdong 512099,China)

机构地区：[1]新疆国际旅行卫生保健中心(乌鲁木齐海关口岸门诊部)医学检验部,新疆乌鲁木齐830000 [2]乌鲁木齐市疾病预防控制中心办公室,新疆乌鲁木齐830023 [3]福建省泉州市第一医院检验科,福建泉州362000 [4]江苏国际旅行卫生保健中心(南京海关口岸门诊部)卫生检验科,江苏南京210004 [5]中国检验检疫研究院卫生检疫研究所,北京100123 [6]韶关海关综合技术服务中心实验室,广东韶关512099

出　　处：《口岸非传统安全学刊》2025年第2期16-19,共4页

基　　金：2023年度海关总署科研项目(2023HK022)。

摘　　要：评估改进后的间接免疫荧光法(Indirect immunofluorescence method,IIFA)在甲型流感病毒IgM抗体检测中的效能提升。引入自动化稀释工作站、磁性纳米颗粒吸附剂、微流控芯片(Microfluidic chip)及人工智能图像分析系统等设备,对传统IIFA进行优化改进。比较改进后的IIFA、传统IIFA及酶联免疫吸附法(Enzyme-linked immunosorbent assay,ELISA)对10547名体检人员进行甲型流感病毒IgM抗体检测,比较阳性率差异。改进后的IIFA阳性率为6.13%,显著高于传统IIFA(5.39%)和酶联免疫法法(5.50%)(P<0.05)。改进后的IIFA显著提高了甲型流感病毒IgM抗体的检出效能,可作为呼吸道感染病原体筛查的优选方案。To evaluate the improved efficacy of indirect immunofluorescence(IIFA)in the detection of influenza A virus IgM antibodies.Automatic dilution workstation,magnetic nanoparticle adsorbent,microfluidic chip and artificial intelligence image analysis system were introduced to optimize and improve the traditional IIFA.The improved IIFA,traditional IIFA and enzyme-linked immunosorbent assay(ELISA)were used to detect influenza A virus IgM in 10547 persons,and the positive rates were compared.The positive rate of improved IIFA was 6.13%,significantly higher than that of traditional IIFA(5.39%)and enzyme-linked immunoassay(5.50%)(P<0.05).The improved IIFA significantly improves the detection efficiency of influenza A virus IgM antibodies,and can be used as a preferred scheme for screening respiratory infection pathogens.

关 键 词：病原体 间接免疫荧光法 甲型流感病毒 IGM抗体 

分 类 号：X18[环境科学与工程—环境科学]