周期性呕吐综合征合并便秘患儿的肠道菌群特征  

Gut microbiota characteristics of children with cyclic vomiting syndrome complicated with constipation

作　　者：李宁宁 李园[2] 宋琳[2] 杜燕燕[2] 王健 季丽娜[2] 徐樨巍[1,2] Li Ningning;Li Yuan;Song Lin;Du Yanyan;Wang Jian;Ji Lina;Xu Xiwei(Beijing Children's Hospital,Capital Medical University,National Center for Children's Health,Beijing 100045,China;Department of Pediatrics,Beijing Tsinghua Changgung Hospital,School of Clinical Medicine,Tsinghua University,Beijing 102218,China)

机构地区：[1]国家儿童医学中心、首都医科大学附属北京儿童医院,100045 [2]清华大学附属北京清华长庚医院儿科、清华大学临床医学院,102218

出　　处：《中国小儿急救医学》2025年第4期286-291,共6页Chinese Pediatric Emergency Medicine

摘　　要：目的探讨周期性呕吐综合征(CVS)合并便秘患儿的肠道菌群分布特征。方法收集2022年6月至2024年1月于北京清华长庚医院就诊的CVS患儿,年龄1~16岁,根据是否合并便秘分为便秘组和普通组。收集患儿的临床资料,并采集粪便样本。使用宏基因组学方法检测两组患儿粪便样本中肠道菌群的丰度、多样性及种类。结果共收集CVS患儿20例,便秘组10例,普通组10例,两组患儿入院年龄、首次发病年龄、体重指数、性别、病情轻重程度、胃镜检查异常情况及腹痛情况等差异无统计学意义。粪便测序共获得470个分类操作单元集,普通组414个,便秘组56个。便秘组肠道菌群丰度及多样性均显著低于普通组。PCoA分析和PCA分析显示,两组患儿的肠道菌群结构存在显著差异。线性判别分析效果大小检测显示,在菌群组成上,普通组以厚壁菌门为主,便秘组以拟杆菌门和放线菌门为主。京都基因与基因组百科全书通路分析显示,便秘组碳代谢通路改变较为明显。结论合并便秘的CVS患儿和未合并便秘的CVS患儿肠道菌群存在显著差异。拟杆菌门、放线菌门等在CVS患儿便秘过程中发挥重要作用。肠道菌群的多样性及其代谢功能可能是CVS患儿便秘发生的病理机制之一。Objective To investigate the characteristics of gut microbiota distribution in children with cyclic vomiting syndrome(CVS)complicated by constipation.Methods The children with CVS,aged from 1 to 16 years,who were admitted to Beijing Tsinghua Changgung Hospital from June 2022 to January 2024,were divided into constipation group and normal group(non-constipation group)according to whether they were complicated with constipation or not.The clinical data and stool samples of children were collect.The abundance,diversity and composition of intestinal flora in fecal samples of two groups were detected by metagenomics sequencing.Results A total of 20 children with CVS were collected,including 10 patients in constipation group and 10 patients in normal group.There were no significant differences in general demographic data between the two groups,including age at admission,age at first onset,body mass index,gender distribution,disease severity,endoscopic findings,and abdominal pain patterns.Microbiome analysis yielded 470 operational taxonomic units(OTUs),with 414 OTUs identified in normal group and 56 OTUs in constipation group.The abundance and diversity of intestinal flora in constipation group were significantly lower than those in normal group.Principal coordinate analysis and principal component analysis indicated significant structural differences in gut microbiota composition between the two groups.LEfSe analysis revealed distinct taxonomic patterns between the two groups,with the normal group demonstrating predominant representation of Firmicutes at the phylum level,while the constipation group showed higher relative abundance of Bacteroidetes and Actinobacteria.KEGG pathway analysis revealed that the carbon metabolism pathways was significantly enriched in the constipation group.Conclusion There are significant differences in intestinal flora between CVS children with and without comorbid constipation.Bacteroides and Actinomycetes play an important role in constipation of children with CVS.The diversity and meta

关 键 词：肠道菌群 周期性呕吐综合征 便秘 儿童 

分 类 号：R725.7[医药卫生—儿科]