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作 者:赵乔锐 李宗芸 ZHAO Qiao-Rui;LI Zong-Yun(College of Life Sciences,Jiangsu Normal University,Xuzhou 221116,China;The Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province,Xuzhou 221116,China)
机构地区:[1]江苏师范大学生命科学学院,徐州221116 [2]江苏省药用植物生物技术重点实验室,徐州221116
出 处:《生命科学》2025年第3期337-346,共10页Chinese Bulletin of Life Sciences
基 金:财政部和农业农村部国家现代农业产业技术体系(甘薯)项目(CARS-10-GW04);国家自然科学基金项目(31771367)。
摘 要:甘薯(Ipomoea batatas(L.)Lam.)具有产量高、适应性强、增殖率高和营养价值高等特点,是全球重要的块根类作物。随着生物技术的不断发展,利用基因工程技术对甘薯进行遗传改良已成为国内外研究的热点。本文对农杆菌介导法、电击法和基因枪法等甘薯遗传转化方法及影响因素进行概述和分析,并综述了近年来甘薯研究在抗非生物胁迫、抗病虫害、抗除草剂、品质改良和块根发育等方面的进展。文章还对基因工程在甘薯遗传改良中的应用前景及相关生物安全性问题进行讨论和展望,以期为今后的甘薯基因工程改良研究工作提供借鉴与参考。Sweetpotato(Ipomoea batatas(L.)Lam.)has the characteristics of high yield,strong adaptability,high proliferation rate and high nutritional value,and is an important root crop in the world.With the continuous development of biotechnology,the genetic improvement of sweetpotato by genetic engineering technology has become a current topic in China and abroad.In this article,we provide an overview and analysis of the genetic transformation methods and influencing factors of sweetpotato,such as agrobacterium-mediated method,electric shock method and gene gun method,and review the latest research progress in the genetic transformation of sweetpotato,including improvement in abiotic stress resistance,antibiotic stress,herbicide resistance,nutritional quality and root development.We also discuss and look forward to the application prospect of genetic engineering in sweetpotato genetic improvement and related biosafety issues,in order to provide reference for the future research on genetic engineering improvement of sweetpotato.
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