Chrysophanol Induces Cell Death and Inhibits Invasiveness through Alteration of Calcium Levels in HepG2 Human Liver Cancer Cells  

作　　者：CHEN Shu-chao CHEN Qiao-wen KO Chih-yuan 

机构地区：[1]Department of Respiratory and Critical Care Medicine,the Second Affiliated Hospital of Fujian Medical University,Quanzhou,Fujian Province,362000,China [2]Department of Clinical Nutrition,the Second Affiliated Hospital of Fujian Medical University,Quanzhou,Fujian Province,362000,China [3]School of Public Health,Fujian Medical University,Fuzhou,350122,China

出　　处：《Chinese Journal of Integrative Medicine》2025年第5期434-440,共7页中国结合医学杂志(英文版)

基　　金：Supported by Academic Funding from the Second Affiliated Hospital of Fujian Medical University(Serial No.BS201902);Quanzhou Science and Technology Project(No.2020N090S,the Malignant Tumor Clinical Medicine Research Center,Quanzhou City,Fujian Province,China)。

摘　　要：Objective:To investigate the effect of chrysophanol,a phytochemical derived from Radix et Rhizoma Rhei on HepG2 liver cancer cells.Methods:HepG2 cell line was treated with different concentrations chrysophanol(0-100μmol/L)for 24 h.The cell counting kit 8 assay was employed to assess cell viability.Intracellular calcium levels were examined using Fluo-4 AM and Mag-fluo-4 AM staining,followed by flow cytometry analysis.Mitochondrial membrane potential was measured with JC-1 assay kit.Additionally,the expressions of key proteins such as p-JNK,Bax,cytochrome c(Cyt C),cleaved caspase-3(cCaspase-3),and caspase-8 were analyzed by Western blot.The inhibitory effects of chrysophanol on the invasion of cells were determined using a Transwell assay.Analysis of invasiveness was conducted by wound healing assay.Results:Chrysophanol significantly reduced the proliferation of HepG2 liver cancer cells by affecting intracellular calcium distribution,diminishing mitochondrial membrane potential,and enhancing the expressions of p-JNK,Bax,Cyt C,cCaspase-3,and caspase-8 in the groups treated with 75 or100μmol/L chrysophanol compared to the control group(P<0.05).Additionally,75 and 100μmol/L chrysophanol exhibited inhibitory effects on cell migration and wound healing.Conclulsion:Chrysophanol demonstrates potential against HepG2 liver cancer cells,suggesting its potential use as a therapeutic agent for liver cancer treatment.

关 键 词：apoptosis calcium CHRYSOPHANOL liver cancer mitochondrial membrane potential Radix et Rhizoma Rhei Chinese medicine 

分 类 号：R285[医药卫生—中药学]