Correlation of IGF2 levels with sperm quality,inflammation,and DNA damage in infertile patients  

作　　者：Jing-Gen Wu Cai-Ping Zhou Wei-Wei Gui Zhong-Yan Liang Feng-Bin Zhang Ying-Ge Fu Rui LiFang Wu Xi-Hua Lin 

机构地区：[1]Department of Reproductive Endocrinology,Women’s Hospital,Zhejiang University School of Medicine,Hangzhou 310006,China [2]Department of Endocrinology and Metabolism,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310006,China

出　　处：《Asian Journal of Andrology》2025年第2期204-210,共7页亚洲男性学杂志(英文版)

基　　金：supported by grants from the National Natural Science Foundation of China(No.82370846);the Zhejiang Provincial Natural Science Foundation of China(No.LY24H070002).

摘　　要：Insulin-like growth factor 2(IGF2)is a critical endocrine mediator implicated in male reproductive physiology.To investigate the correlation between IGF2 protein levels and various aspects of male infertility,specifically focusing on sperm quality,inflammation,and DNA damage,a cohort of 320 male participants was recruited from the Women’s Hospital,Zhejiang University School of Medicine(Hangzhou,China)between 1^(st) January 2024 and 1^(st) March 2024.The relationship between IGF2 protein concentrations and sperm parameters was assessed,and Spearman correlation and linear regression analysis were employed to evaluate the independent associations between IGF2 protein levels and risk factors for infertility.Enzyme-linked immunosorbent assay(ELISA)was used to measure IGF2 protein levels in seminal plasma,alongside markers of inflammation(tumor necrosis factor-alpha[TNF-α]and interleukin-1β[IL-1β]).The relationship between seminal plasma IGF2 protein levels and DNA damage marker phosphorylated histone H2AX(γ-H2AX)was also explored.Our findings reveal that IGF2 protein expression decreased notably in patients with asthenospermia and teratospermia.Correlation analysis revealed nuanced associations between IGF2 protein levels and specific sperm parameters,and low IGF2 protein concentrations correlated with increased inflammation and DNA damage in sperm.The observed correlations between IGF2 protein levels and specific sperm parameters,along with its connection to inflammation and DNA damage,underscore the importance of IGF2 in the broader context of male reproductive health.These findings lay the groundwork for future research and potential therapeutic interventions targeting IGF2-related pathways to enhance male fertility.

关 键 词：ASTHENOSPERMIA DNA damage IGF2 INFLAMMATION sperm quality teratospermia 

分 类 号：R69[医药卫生—泌尿科学]