The SPARC-related modular calcium binding 1(Smoc1)regulated by androgen is required for mouse gubernaculum development and testicular descent  

作　　者：Zhi-Yi Zhao Yong Siow Ling-Yun Liu Xian Li Hong-Liang Wang Zhen-Min Lei 

机构地区：[1]Department of Andrology,The First Hospital of Jilin University,Changchun 130021,China [2]Department of OB/GYN,University of Louisville School of Medicine,Louisville,KY 40202,USA

出　　处：《Asian Journal of Andrology》2025年第1期44-51,共8页亚洲男性学杂志(英文版)

基　　金：supported in part by the Department of OB/GYN research funds(University of Louisville,Louisville,KY,USA);Jilin Province Health Technology Capability Enhancement funds(No.2022JC055).

摘　　要：Testicular descent occurs in two consecutive stages:the transabdominal stage and the inguinoscrotal stage.Androgens play a crucial role in the second stage by influencing the development of the gubernaculum,a structure that pulls the testis into the scrotum.However,the mechanisms of androgen actions underlying many of the processes associated with gubernaculum development have not been fully elucidated.To identify the androgen-regulated genes,we conducted large-scale gene expression analyses on the gubernaculum harvested from luteinizing hormone/choriogonadotropin receptor knockout(Lhcgr KO)mice,an animal model of inguinoscrotal testis maldescent resulting from androgen deficiency.We found that the expression of secreted protein acidic and rich in cysteine(SPARC)-related modular calcium binding 1(Smoc1)was the most severely suppressed at both the transcript and protein levels,while its expression was the most dramatically induced by testosterone administration in the gubernacula of Lhcgr KO mice.The upregulation of Smoc1 expression by testosterone was curtailed by the addition of an androgen receptor antagonist,flutamide.In addition,in vitro studies demonstrated that SMOC1 modestly but significantly promoted the proliferation of gubernacular cells.In the cultures of myogenic differentiation medium,both testosterone and SMOC1 enhanced the expression of myogenic regulatory factors such as paired box 7(Pax7)and myogenic factor 5(Myf5).After short-interfering RNA-mediated knocking down of Smoc1,the expression of Pax7 and Myf5 diminished,and testosterone alone did not recover,but additional SMOC1 did.These observations indicate that SMOC1 is pivotal in mediating androgen action to regulate gubernaculum development during inguinoscrotal testicular descent.

关 键 词：ANDROGEN androgen receptor GUBERNACULUM Smoc1 testis descent 

分 类 号：R697[医药卫生—泌尿科学]