喹硫平通过PI3K/AKT通路抑制A549细胞增殖、迁移和侵袭  

Quetiapine inhibits the proliferation,migration and invasion of A549 cells through PI3K/AKT pathway

作　　者：唐浩 朱世达 袁术杰 陈凯 黎源鑫 梁春涛 王洪凯 TANG Hao;ZHU Shi-Da;YUAN Shu-Jie(Department of Orthopedics,the Second Afiliated Hospital of Guilin Medical University,Guilin 541199,Guangxi,China)

机构地区：[1]桂林医学院第二附属医院骨科,广西桂林541199

出　　处：《中国老年学杂志》2025年第8期1888-1893,共6页Chinese Journal of Gerontology

基　　金：国家自然科学基金地区项目(81960172);广西自然科学基金面上项目(2020GXNSFAA238014);广西医疗卫生重点培育学科建设项目(桂卫科教发[2022]4号)。

摘　　要：目的 研究喹硫平对肺癌A549细胞增殖、迁移和侵袭的作用及分子调控机制。方法 使用不同浓度(0、1、50、100μmol/L)喹硫平处理A549细胞后,用噻唑蓝(MTT)实验检测A549细胞增殖活性;平板克隆实验检测A549细胞集落形成能力;划痕修复实验和Transwell侵袭实验检测喹硫平对A549细胞迁移和侵袭的影响;流式细胞术检测喹硫平对A549细胞凋亡的影响;Western印迹检测喹硫平对A549细胞磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)信号通路和凋亡相关蛋白表达的影响。结果 与0μmol/L喹硫平组相比,50和100μmol/L喹硫平组A549细胞的增殖活性、集落形成能力、迁移能力及侵袭能力明显降低(P<0.05,P<0.01);与0μmol/L喹硫平组相比,100μmol/L喹硫平组A549细胞凋亡比例、Cleaved-caspase3蛋白表达明显升高,p-PI3K、p-AKT蛋白表达明显降低(P<0.05)。结论 喹硫平通过PI3K/AKT通路促进A549细胞凋亡进而抑制A549细胞增殖、迁移和侵袭。ObjectiveeTo study the effects of quetiapine on proliferation,migration and invasion of lung cancer A549 cells and its molecular regulatory mechanism.Methods After different concentrations of quetiapine(0,1,50,100μmol/L)were used to treat A549 cells,thiazolyl blue(MTT)experiment was used to detect the activity of A549 cell proliferation.The colony formation ability of A549 cells was detected by plate cloning assay.Scratch repair experiment and Transwell migration assay were used to detect the effects of quetiapine on the migration and invasion of A549 cells.Flow cytometry was used to detect the effect of quetiapine on A549 cell apoptosis.Western blot was used to detect the effects of quetiapine on the A549 cell phosphatidyl inositol 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway and apoptosis related protein expressions.Results Compared with Oμmol/L quetiapine group,the proliferation activity,colony formation ability,migration and invasion ability of A549 cells in 50 and 100μmol/L quetiapine groups were significantly decreased(P<0.05,P<0.01).Compared with the 0μmol/L quetiapine group,the apoptosis ratio of A549 cells,Cleaved-caspase3 protein expression were significantly increased,p-PI3K and p-AKT protein expressions were significantly decreased in the 100μmol/L quetiapine group(P<0.05).Conclusions Quetiapine could promote apoptosis in A549 cells through PI3K/AKT pathway,thereby inhibiting proliferation,migration and invasion of A549 cells.

关 键 词：喹硫平 非小细胞肺癌 磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)通路 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤]