miR-204-3p干预二氧化硅粉尘诱导的大鼠矽肺上皮间质转化进程  

miR-204-3p affects silica dust-induced epithelial mesenchymal transition in a rat silicosis model

作　　者：俞静 陈芳 胡文选 皮洋阳 张玺 王露凝 赵萍 王发选[1] YU Jing;CHEN Fang;HU Wenxuan;PI Yangyang;ZHANG Xi;WANG Luning;ZHAO Ping;WANG Faxuan(School of Public Health,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学公共卫生学院,银川750004

出　　处：《中国比较医学杂志》2025年第3期40-47,共8页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(82460634,82060584)。

摘　　要：目的 探索miR-204-3p在矽肺上皮间质转化(epithelial-mesenchymal transition, EMT)中的作用,阐明miR-204-3p通过调控二氧化硅粉尘诱导的大鼠肺泡EMT过程进而影响矽肺纤维的机制。方法 取40只SD大鼠随机均分为4组:对照(Control)、矽肺(Silicosis)、AAV-Control和AAV-miR-204-3p组。苏木精-伊红(HE)、Masson染色进行大鼠肺组织损伤的病理学观察;实时荧光逆转录定量PCR(RT-qPCR)法检测各组大鼠肺组织中miR-204-3p和EMT标志物基因相对表达水平;Western blot法检测各组大鼠肺组织中EMT相关标志物蛋白表达水平。结果 与对照组相比,矽肺组肺泡结构损伤,肺间隔间质纤维化,间充质标志物表达升高(P<0.05,P<0.01,P<0.001);与AAV-Control组相比,AAV-miR-204-3p组肺泡结构较完整,EMT过程缓解,纤维化得到改善,间充质标志物表达降低(P<0.05,P<0.01,P<0.001)。结论 游离的二氧化硅粉尘可诱导大鼠肺组织EMT,过表达miR-204-3p对游离二氧化硅粉尘所致大鼠EMT过程具有干预作用,有望影响矽肺纤维化进程。Objective To explore the role of miR-204-3p in silicosis and to elucidate the mechanism by which it affects silicosis fibers by regulating silica dust-induced alveolar epithelial-mesenchymal transition(EMT) in rats. Methods Forty SD rats were divided randomly into 4 groups: Control, Silicosis, AAV-Control, and AAV-miR-204-3p groups. The pathology of lung tissue damage was detected by hematoxylin and eosin(HE)and Masson staining. Relative expression levels of miR-204-3p and EMT marker genes in lung tissues from rats in each group were analyzed by real-time fluorescence reverse transcription quantitative PCR(RT-qPCR), and protein expression levels of EMT-related markers in lung tissues were detected by Western blot. Results The alveolar structure was damaged, the lung septa showed interstitial fibrosis, and expression levels of mesenchymal markers were elevated in the Silicosis group compared with the Control group(P<0.05, P<0.01, P<0.001). The alveolar structure was more complete, the EMT process was alleviated, fibrosis was improved, and mesenchymal marker expression was reduced in the AAV-miR-204-3p group compared with the AAV-Control group(P<0.05, P<0.01, P<0.001). Conclusions Free silica dust induces EMT in rat lung tissue. Overexpression of miR-204-3p can attenuate the EMT process induced by free silica dust in rats, and may thus affect silicosis fibrosis.

关 键 词：矽肺 二氧化硅 EMT ECM miR-204-3p 

分 类 号：R-33[医药卫生]