两种造模方法建立弱精子症伴高SDF小鼠模型的比较研究  

Comparative experimental study of sodium benzoate and corticosterone in a mouse model of asthenozoospermia with high sperm DNA fragmentation

作　　者：卢宗林 赵海洋 王晖[3] 王鑫[1] 孙自学[5] LU Zonglin;ZHAO Haiyang;WANC Hui;WANC Xin;SUN Zixue(the Second Clinical Medical College,Henan University of Chinese Medicine,Zhengzhou 450008,China;Beijing University of Traditional Chinese Medicine Dongzhimen Hospital Luoyang Hospital,Luoyang 471000;State Key Laboratory of Reproductive Medicine and Ofspring Health,Department of Histology and Embryology,School of Basic Medical Sciences,Nanjing Medical University,Nanjing 211166;Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine,Ministry of Education,Shanghai University of Traditional Chinese Medicine,Shanghai 201203;Henan Provincial Hospital of Traditional Chinese Medicine,Zhengzhou 450000)

机构地区：[1]河南中医药大学第二临床医学院,郑州450008 [2]北京中医药大学东直门医院洛阳医院,河南洛阳471000 [3]南京医科大学生殖医学与子代健康全国重点实验室,基础医学院组织胚胎学系,南京211166 [4]上海中医药大学中药现代制剂技术教育部工程研究中心,上海201203 [5]河南省中医院,郑州450000

出　　处：《中国比较医学杂志》2025年第3期48-57,共10页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(82274536);洛阳市科技发展计划项目(2022017Y)。

摘　　要：目的通过激素皮质酮(corticosterone,CORT)激素和化合物苯甲酸钠(sodium benzoate,NaB)诱导构建弱精子症(asthenozoospermia,AZS)伴高精子DNA碎片化(sperm DNA fragmentation,SDF)小鼠模型,探索AZS伴高SDF动物模型的制备方法。方法将50只3周龄雄性ICR小鼠随机分为CORT处理组30只,NaB处理组20只。CORT处理组分为6组,分别为饮水高(500μg/mL)、中(200μg/mL)、低(10μg/mL)剂量组、饮水对照组、注射造模组(40 mg/kg)和注射对照组(生理盐水),每组5只,连续造模50 d。NaB处理组分为4组,分别为灌胃高(500 mg/kg)、中(300 mg/kg)、低(100 mg/kg)剂量组和对照组(生理盐水),每组5只,连续造模50 d。造模中观察各组小鼠生理状态并持续记录体质量变化,结束后取小鼠附睾尾部精子观察精子运动能力和精子DNA碎片化指数(DNA fragmentation index,DFI)。结果注射造模组体质量变化率出现下降趋势,与注射对照组相比无显著性差异(P>0.05),灌胃高剂量组体质量变化率下降,与对照组相比差异显著(P<0.05);注射造模组前向运动精子百分率出现下降趋势,与注射对照组相比无显著性差异(P>0.05),NaB灌胃高剂量组前向运动精子百分率下降,与对照组相比差异显著(P<0.05);注射造模组DFI出现升高趋势,与注射对照组相比无显著性差异(P>0.05),灌胃高剂量组DFI升高,与对照组相比差异显著(P<0.05)。结论NaB 500 mg/(kg·d)剂量持续50 d灌胃造模是构建AZS伴高SDF动物模型较为理想的方法。Objective To prepare a mouse model of asthenozoospermia(AZS) with high sperm DNA fragmentation(SDF) using corticosterone(CORT) and sodium benzoate(NaB). Methods Fifty 3-week-old male ICR mice were divided randomly into CORT-treated(n=30) and NaB-treated(n=20) groups. The CORT group was further divided into the following six groups(n=5 per group): high CORT(500 μg/mL), medium CORT(200 μg/mL), and low CORT(10 μg/mL) drinking water group, drinking water control group, CORT injection(40 mg/kg) group, and injection control group(normal saline). The animals were modeled continuously for 50 d. Mice in the NaB group were further divided into four groups(n=5 per group): high NaB(500 mg/kg), medium NaB(300 mg/kg), and low NaB(100 mg/kg) gavage groups, and control group(normal saline). The animals were modeled continuously for 50 d. The physiological state of the mice in each group was observed and mass changes were recorded continuously. The sperm motility capacity and DNA fragmentation index(DFI) of the sperm were observed from the tail of the epididymis after the end of the modeling. Results The rate of mass change in the CORT-injection moding group showed a downward trend. There was no significant difference(P>0.05) in the high NaB gavage group, and the rate of body mass change in the high NaB gavage group was significantly decreased compared with the control group(P<0.05). The percentages of forward motility sperm were significantly decreased in the CORT injection group(P>0.05) and the percentage in the high NaB gavage group(P<0.05), compared with the control group. The DFI was increased in the CORT injection group compared with the control group, but the difference was not significant(P>0.05), and the DFI in the high NaB gavage group was significantly increased compared with the control group(P<0.05). Conclusions Intragastric gavage with NaB 500 mg/(kg·d) for 50 d is an ideal method for constructing an animal model of AZS with high SDF.

关 键 词：弱精子症伴高SDF 小鼠模型 苯甲酸钠 皮质酮 

分 类 号：R-33[医药卫生]