适用于蛛网膜下腔阻滞相关研究的背侧入路腰交感神经解剖新模型  

A novel anatomical model of the lumbar sympathetic nerve for subarachnoid-related studies via a dorsal approach

作　　者：顾英花 刘昕 李艳[1] 刘文勋[2] 马维义 叶青山[2] GU Yinghua;LIU Xin;LI Yan;LIU Wenxun;MA Weiyi;YE Qingshan(Third Clinical School of Medicine,Ningxia Medical University,Yinchuan 750000,China;Department of Anesthesiology,Ningxia Hui Autonomous Region People's Hospital,Yinchuan 750000)

机构地区：[1]宁夏医科大学第三临床医学院,银川750000 [2]宁夏回族自治区人民医院麻醉科,银川750000

出　　处：《中国比较医学杂志》2025年第3期100-108,共9页Chinese Journal of Comparative Medicine

基　　金：宁夏回族自治区重点研发计划项目(2022BEG03114)。

摘　　要：目的 通过背侧入路建立损伤较小且适用于蛛网膜下腔阻滞相关研究的交感神经解剖和监测新模型。方法 利用SD大鼠建立传统腹侧入路模型以及背侧入路新模型,观察两种模型成模时间。通过蛛网膜下腔注射布比卡因观察血压(blood pressure, BP)、心率(heart rate, HR)、腰交感神经变化百分比(percentage change in lumbar sympathetic nerve electrical activity, LSNA change%)、去甲肾上腺素(norepinephrine, NE)、一氧化氮(nitric oxide, NO)的含量评估新模型的稳定性。结果 (1)建立新模型:与传统腹侧入路(VA)组的模型方法相比,背侧入路(DA)组解剖新模型成模时间更短(P<0.0001)。(2)评估新模型:与盐水(NS)组相比,蛛网膜下腔注射布比卡因后5 min(T_(2))和10 min(T_(3))时平均动脉压(mean arterial pressure, MAP)和收缩压(systolic blood pressure, SBP)降低(P<0.05);LSNA change%变化显著(P<0.05);注射布比卡因10 min(T_3)时NE的浓度降低(P<0.05)。结论 构建了适用于蛛网膜下腔相关研究的背侧入路腰交感神经解剖新模型;并成功用于蛛网膜下腔阻滞麻醉对腰交感神经活动影响的研究。Objective The study attempted to establish a less invasive model for dissecting and monitoring the sympathetic nerve in rats via a dorsal approach for subarachnoid block-related studies. Methods A traditional abdominal approach model and a new dorsal approach model were established in SD rats, and the modeling time of the two models was observed. The stability of the new model was evaluated by measuring blood pressure(BP), heart rate(HR), percentage change in lumbar sympathetic nerve activity(LSNA change%), norepinephrine(NE), and nitric oxide(NO)content after subarachnoid injection of bupivacaine. Results(1)Building a new model: the time required to create new models for the dorsal approach(DA)group was shorter than that for the traditional abdominal approach(VA)group, as shown by the result(P<0.0001).(2)Evaluation of the new model: compared with the NS group, the MAP and SBP were lower at T_2(5 min after injection of bupivacaine into the subarachnoid space)and T_3(10 min)(P<0.05);the LSNA change% was significantly different(P<0.05);the concentration of NE was lower at T_3(P<0.05). Conclusions The study presents a novel lumbar sympathetic anatomy model using the dorsal approach for subarachnoid-related investigations, which was effectively employed to examine the impact of subarachnoid block anesthesia on lumbar sympathetic nerve activity.

关 键 词：腰交感神经 模型 蛛网膜下腔阻滞 

分 类 号：R-33[医药卫生]