circTRRAP通过miR-194-3p调控KLK10促进结直肠癌进展的机制  

Mechanism of circTRRAP regulating KLK10 through miR-194-3p to promote colorectal cancer progression

作　　者：于德明[1] 王振军[2] 陈泓宇 陈志磊 杨磊[2] 李向南[2] 李南[1] YU Deming;WANG Zhenjun;CHEN Hongyu;CHEN Zhilei;YANG Lei;LI Xiangnan;LI Nan(Department of General Surgery,Beijing Luhe Hospital,Capital Medical University,Beijing101199,China;Department of General Surgery,Beijing Chao-Yang Hospital,Capital Medical University,Beijing100020,China)

机构地区：[1]首都医科大学附属北京潞河医院普外科,北京101199 [2]首都医科大学附属北京朝阳医院普外科,北京100020

出　　处：《中国医药导报》2025年第9期1-6,共6页China Medical Herald

基　　金：国家自然科学基金青年科学基金资助项目(82103361)。

摘　　要：目的探讨环状核糖核酸转化/转录域关联蛋白(circTRRAP)通过miR-194-3p调控KLK10促进结直肠癌(CRC)进展的机制。方法利用miRanda软件及TCGA数据库筛选circTRRAP的靶miRNA。分析miR-194-3p高表达组与miR-194-3p低表达组的生存时间差异。采用双萤光素酶报告基因检测对靶miRNA进行验证。利用体外细胞功能实验分析miR-NC组、miR-194-3p mimics组、circTRRAP+miR-NC组、circTRRAP+miR-194-3p mimics组结直肠癌细胞增殖力、迁移力和侵袭力,利用蛋白质印迹实验分析circTRRAP组、NC组、circTRRAP+miR-NC组、circTRRAP+miR-194-3p mimics组KLK10蛋白表达的变化。结果miR-194-3p可能是circTRRAP的靶miRNA,且miR-194-3p高表达组的生存时间长于miR-194-3p低表达组(P<0.05)。circTRRAP组的细胞增殖力、迁移力和侵袭力高于miR-194-3p mimics组(P<0.05)。circTRRAP组的KLK10蛋白表达高于miR-194-3p mimics组(P<0.05)。结论circTRRAP通过miR-194-3p调控KLK10促进CRC的进展。Objective To explore the mechanism of circular RNA transformation/transcription domain associated protein(circTRRAP)in colorectal cancer(CRC)by regulating KLK10 through miR-194-3p.Methods The target mirnas of circTRRAP were screened using miRanda software and the TCGA database.The survival time difference between miR-194-3p high expression group and miR-194-3p low expression group was analyzed.The target miRNA was verified by double luciferase reporter gene assay.The proliferation,migration,and invasion of colorectal cancer cells in miR-NC group,miR-194-3p mimics group,circTRRAP+miR-NC group,and circTRRAP+miR-194-3p mimics group were analyzed by in vitro cell function experiment.The changes of KLK10 protein expression in circTRRAP group,NC group,circTRRAP+miR-NC group,and circTRRAP+miR-194-3p mimics group were analyzed by Western blot.Results miR-194-3p may be the target miRNA of circTRRAP,and the survival time of miR-194-3p high expression group was longer than that of miR-194-3p low expression group(P<0.05).Cell proliferation,migration,and invasion in circTRRAP group were higher than those in miR-194-3p mimics group(P<0.05).KLK10 protein expression in circTRRAP group was higher than that in miR-194-3p mimics group(P<0.05).Conclusion circTRRAP may facilitate the progression of CRC by regulating KLK10 through the miR-194-3p.

关 键 词：结直肠癌 circTRRAP miR-194-3p KLK10 

分 类 号：R735.3[医药卫生—肿瘤]