杂色曲霉产葡聚糖内切酶条件优化及酶学性质初步研究  

Optimizing conditions for endoglucanase from Aspergillus versicolor and preliminary study on its enzymatic properties

作　　者：付跃 陆桂雅 黄玲胜 易群 梁彩荣 罗奉奉 FU Yue;LU Guiya;HUANG Lingsheng;YI Qun;LIANG Cairong;LUO Fengfeng(College of Chemical and Biological Engineering,Hechi University,Hechi,Guangxi 546300,China;Guangxi Colleges Universities Key Laboratory of Exploitation and Utilization of Microbial and Botanical Resources,Hechi,Guangxi 546300,China;Agricultural Biotechnology Application Research Center,Hechi University,Hechi,Guangxi 546300,China)

机构地区：[1]河池学院化学与生物工程学院,广西河池546300 [2]微生物及植物资源开发利用广西高校重点实验室,广西河池546300 [3]河池学院农业生物技术应用研究中心,广西河池546300

出　　处：《中国饲料》2025年第9期45-50,共6页China Feed

基　　金：广西高校中青年教师科研基础能力提升项目(2020KY15018);河池学院科研项目(2021XJZD006);广西自然科学基金(2020GXNSFAA297218)。

摘　　要：本研究采用单因素试验和正交试验对杂色曲霉产葡聚糖内切酶发酵条件进行优化,并对其酶学性质进行初步探究,旨在为木质纤维素类生物质在饲料领域中的应用提供数据支撑。优化后的发酵条件为:蔗渣与麸皮比例4:3,硫酸铵添加量2%,发酵温度28℃,发酵时间4 d,在此条件下葡聚糖内切酶酶活为37.92 U/mL,比优化前提高了1.64倍。酶学性质研究结果表明:杂色曲霉产葡聚糖内切酶的最适温度为55℃,在温度35~55℃内是稳定的。最适pH为4,在pH为4~6内是稳定的。Ca^(2+)、Ba^(2+)对葡聚糖内切酶酶活有促进作用,Co^(2+)和Mg^(2+)具有一定的抑制作用。For provide data support for the application of lignocellulosic biomass in the field of feed,optimizing conditions for endoglucanase from Aspergillus versicolor by single factor experiment and orthogonal experiment and preliminary study on its enzymatic properties.The results showed that the optimal conditions were as follows:the ratio of bagasse to bran was 4:3,the additive amount of(NH4)2SO4 was 2%,the fermentation temperature was 28℃,the fermentation time was 4 d.Under the optimal conditions,the enzyme activity was 37.92 U/mL,which was 1.64 times higher than before optimization.The results of enzymatic properties study showed that the endoglucanase from Aspergillus versicolor had maximum activity at 55℃and optimum pH at 4,respectively,and the enzyme had good stability at 35~55℃and pH 4.0~6.0.The metal ions Ca^(2+),Ba^(2+)could promote the endoglucanase activity,while metal ions Co^(2+),Mg^(2+)could inhibit the endoglucanase activity.

关 键 词：杂色曲霉 葡聚糖内切酶 产酶条件优化 酶学性质 

分 类 号：S816.3[农业科学—饲料科学]