p-mTOR、p-S6K在鼻腔鼻窦内翻性乳头状瘤中的高表达及临床价值  

Clinical value of highly expressed p-mTOR and p-S6K in sinonasal inverted papilloma

作　　者：毕恒 谢佳 龚辉成 BI Heng;XIE Jia;GONG Huicheng(Department of Otorhinolaryngology&Head and Neck Surgery,Guangzhou Twelfth People's Hospital(Guangzhou Otolaryngology-head and Neck Surgery Hospital)/Department of Otorhinolaryngology&Head and Neck Surgery,The Affiliated Guangzhou Twelfth People's Hospital,Guangzhou Medical University/Institute of Otolaryngology-Head and Neck Surgery,Guangzhou Medical University,Guangzhou 510620,Guangdong,China)

机构地区：[1]广州市第十二人民医院(广州市耳鼻咽喉头颈外科医院)耳鼻咽喉头颈外科/广州医科大学附属市十二人民医院耳鼻咽喉头颈外科/广州医科大学耳鼻咽喉头颈外科研究所,广东广州510620

出　　处：《山东大学耳鼻喉眼学报》2025年第2期25-34,共10页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：广州市科技计划项目(2023A03J0973、2024A03J0492);广东省医学科研基金项目(B2024258)。

摘　　要：目的为筛选有助于鉴别鼻息肉、鼻腔鼻窦内翻性乳头状瘤(sinonasal inverted papilloma,SNIP)的分子标志物提供方向,探讨mTOR通路抑制剂在SNIP治疗中的应用理论依据。方法通过实验分析SNIP中磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)、磷酸化核糖体蛋白S6激酶(p-S6K)较中鼻甲黏膜表达的差异,从而为验证mTOR通路介导SNIP的发病提供实验依据。采用免疫组织化学染色方法检测实验组与对照组p-mTOR、p-S6K的表达情况,运用SPSS 23.0进性统计学分析表达差异,同时采用新鲜标本及瘤旁标本进行免疫蛋白印迹实验对结果加以验证。结果64例SNIP石蜡包埋组织标本中p-mTOR、p-S6K的阳性表达率分别为85.94%、81.25%,对照组32例中鼻甲黏膜中p-mTOR、p-S6K的阳性表达率分别为34.38%、40.63%,两组之间经过统计学分析处理显示差异具有统计学意义(χ^(2)=26.400,P<0.01;χ^(2)=16.103,P<0.01);免疫蛋白印迹实验亦证实SNIP实验组与瘤旁对照标本p-mTOR、p-S6K表达存在显著差异。结论p-mTOR、p-S6K在SNIP的发病机制中发挥重要作用,mTOR通路的过度激活是促进SNIP发病的重要机制。Objective In order to provide directions for screening molecular markers that can help identify nasal polyps and sinonasal inverted papilloma(SNIP),and to provide a theoretical basis for exploring the application of mTOR pathway inhibitors in the treatment of SNIP.Methods The differences in the expression of phosphorylated mammalian target of rapamycin(p-mTOR)and phosphorylated ribosomal protein S6 kinase(p-S6K)in SNIP compared with that of the middle turbinate mucosa were analyzed experimentally,which provided an experimental basis for verifying that the mTOR pathway mediates the pathogenesis of SNIP.Immunohistochemical staining was used to detect the expression of p-mTOR and p-S6K in the experimental group and the control group,and the differences in expression were analyzed using SPSS23.0 progressive statistics,and the results were verified by Western blotting(WB)experiments using fresh SNIP specimens and paraneoplastic specimens.Results The positive expression rates of p-mTOR and p-S6K in paraffin-embedded tissue specimens of 64 cases of SNIP were 85.94%and 81.25%,respectively,and those of p-mTOR and p-S6K in the mucosa of 32 cases of middle turbinate in the control group were 34.38%and 40.63%,respectively,and the differences were statistically significant after the statistical analysis process(χ^(2)=26.400,P<0.01;χ^(2)=16.103,P<0.01);WB experiments also confirmed that there were significant differences in the expression of p-mTOR and p-S6K between the SNIP experimental group and the paraneoplastic control group.Conclusion p-mTOR and p-S6K play an important role in the pathogenesis of SNIP,and the over-activation of mTOR pathway is an important mechanism to promote the pathogenesis of SNIP.

关 键 词：鼻腔鼻窦内翻性乳头状瘤 免疫组织化学染色 P-MTOR p-S6K 蛋白表达 

分 类 号：R739.62[医药卫生—肿瘤]