复硝酚钠对铁皮石斛中黄酮含量和抗氧化活性的影响  

Effect of sodium nitrophenolate on flavonoid content and antioxidant activity in Dendrobium officinale

作　　者：孙凤婷 王旭 韩新雨 许振岚[1] 吴声敢[1] 黄浩 汤涛[1] 盛清[2] 王强[1] 沈国强 赵学平[1] SUN Fengting;WANG Xu;HAN Xinyu;XU Zhenlan;WU Shenggan;HUANG Hao;TANG Tao;SHENG Qing;WANG Qiang;SHEN Guoqiang;ZHAO Xueping(Key Laboratory for Pesticide Residue Detection,Ministry of Agriculture and Rural Affairs,Institute of Agro-Product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou 310018,China;Faculty of Materials Science and Chemistry,China University of Geosciences,Wuhan 430078,China;School of Food Science and Engineering,Hainan University,Haikou 570228,China;Institute of Resources and Environment,Shaoxing Academy of Agricultural Sciences,Shaoxing 312003,Zhejiang,China)

机构地区：[1]浙江省农业科学院农产品质量安全与营养研究所,农业农村部农药残留检测重点实验室,浙江杭州310021 [2]浙江理工大学生命科学与医药学院,浙江杭州310018 [3]中国地质大学材料与化学学院,湖北武汉430078 [4]海南大学食品科学与工程学院,海南海口570228 [5]绍兴市农业科学研究院资源与环境研究所,浙江绍兴312003

出　　处：《浙江农业学报》2025年第4期934-942,共9页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(21806143)。

摘　　要：黄酮类化合物是铁皮石斛最主要的功效成分之一。为探究复硝酚钠对铁皮石斛中黄酮含量的影响,采用茎叶喷雾的方法,用不同剂量的复硝酚钠处理一年生铁皮石斛苗,以清水处理为对照,在末次施药后0、7、15、22、30、45、60、90、120、150、180 d(采收期)取样,测定总黄酮含量和抗氧化活性,并通过超高效液相色谱串联质谱(UPLC-MS/MS)结合QuEChERS的方法定量检测铁皮石斛中17种黄酮化合物。结果表明,施用复硝酚钠能够提高铁皮石斛茎中总黄酮含量和抗氧化活性。同时,复硝酚钠对铁皮石斛茎中不同黄酮化合物含量的影响不同。施用复硝酚钠对柚皮素、圣草酚等13种黄酮化合物的积累起到促进作用。相关性分析表明,抗氧化活性与总黄酮含量呈正相关。因此,施用复硝酚钠可以提高铁皮石斛的总黄酮含量,从而提高其抗氧化活性。Flavonoids are one of the most significant bioactive constituents in Dendrobium officinale.To investigate the effects of sodium nitrophenolate on flavonoid content in D.officinale,one-year-old seedlings were treated with different concentrations of sodium nitrophenolate through foliar spraying,using distilled water as the control.Sampling was conducted at 0,7,15,22,30,45,60,90,120,150,and 180 days(harvest period)after the final application.Total flavonoid content and antioxidant activity were measured,while 17 flavonoid compounds were quantitatively analyzed using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)coupled with the QuEChERS method.Results demonstrated that sodium nitrophenolate application enhanced both total flavonoid content and antioxidant activity in D.officinale stems.Furthermore,sodium nitrophenolate exhibited differential effects on the accumulation of individual flavonoid compounds.Specifically,it promoted the accumulation of 13 flavonoids including naringenin and eriodictyol.Correlation analysis revealed a positive relationship between antioxidant activity and total flavonoid content.These findings indicate that sodium nitrophenolate application can effectively improve the total flavonoid content in D.officinale,thereby enhancing its antioxidant activity.

关 键 词：铁皮石斛 复硝酚钠 黄酮 超高效液相色谱串联质谱 抗氧化活性 

分 类 号：S567.239[农业科学—中草药栽培]