福鼎白茶提取物的抗氧化和抗炎生物活性研究  

Investigation into the antioxidant and anti-inflammatory bioactivities of fuding white tea extracts

作　　者：张梓祺 褚燕 孔丁峰 杨三秀 黄婉婷 刘辉 陈欢 洪圣葆 陈淑娴 王琴 杜立永 ZHANG Ziqi;CHU Yan;KONG Dingfeng;YANG Sanxiu;HUANG Wanting;LIU Hui;CHEN Huan;HONG Shengbao;CHEN Shuxian;WANG Qin;DU Liyong(School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,Jiangsu,China;Shanghai Chunzhibao Biotechnology Co.,Ltd,Shanghai 201100,Shaanxi,China;Wuxi Institute of Inspection,Testing and Certification,Wuxi,214125 China)

机构地区：[1]江南大学化学与材料工程学院,江苏无锡214122 [2]上海春之葆生物科技有限公司,上海201100 [3]无锡质量检验认证研究院,江苏无锡214125

出　　处：《中国化妆品科学》2025年第1期122-129,共8页

基　　金：江苏省市场监管局科技计划项目(KJ2024018)。

摘　　要：茶类特色植物的开发使我国植物化妆品具有独特的优势,为评估白茶提取物作为化妆品原料的抗氧化和抗炎的生物活性潜力,以白毫银针(福鼎白茶中的一种)为原料,采用常规水提法,以黄酮、总酚、氨基酸、生物碱含量为指标,通过四因素三水平的正交实验得到白茶的最佳提取工艺为料液比1:20,温度60℃,提取时间2 h,提取次数3次。为了筛选出等级较高的白茶品种,应用最佳提取工艺提取白牡丹、正安白茶、兴山白茶。通过比较发现,白毫银针的综合指标最优,白毫银针提取液的黄酮含量为45.99 mg/g,总酚含量为66.49 mg/g,氨基酸含量为11.46mg/g,生物碱含量为52.06mg/g。当冻干粉水溶液浓度分别达到30μg/mL和20μg/mL时,ABTS+自由基清除率达到100%。巨噬细胞炎症模型发现白毫银针提取液具有抗炎活性,可显著降低巨噬细胞RAW264.7中的炎症因子IL-6水平,本研究深化了对福鼎白茶作为化妆品功能性成分的科学认知。The development of characteristic plants such as tea has endowed China's botanical cosmetics with unique advantages.To evaluate the antioxidant and anti-inflammatory bioactive potential of white tea extracts as cosmetic raw materials,Baihao Yinzhen(a variety of Fuding white tea)was selected as the raw material.Using conventional water extraction and targeting flavonoid,total phenol,amino acid,and alkaloid contents as indicators,the optimal extraction process was determined through a three-level orthogonal experiment with four factors.The results showed that the best extraction conditions were a solid-liquid ratio of 1:20,temperature of 60°C,extraction time of 2 hours,and 3 extraction cycles.To screen higher-grade white tea varieties,the optimized process was applied to extract Bai Mudan,Zheng'an white tea,and Xingshan white tea.Comparative analysis revealed that Baihao Yinzhen exhibited the highest comprehensive indices:its extract contained 45.99 mg/g flavonoids,66.49 mg/g total phenols,11.46 mg/g amino acids,and 52.06 mg/g alkaloids.When the concentrations of freeze-dried powder aqueous solution reached 30μg/mL and 20μg/mL,the ABTS+radical scavenging rate achieved 100%.Additionally,a macrophage inflammation model demonstrated that Baihao Yinzhen extract possessed anti-inflammatory activity,significantly reducing IL-6 levels in RAW264.7 macrophages.This study advances the scientific understanding of Fuding white tea as a functional ingredient in cosmetics.

关 键 词：福鼎白茶 抗氧化活性 抗炎活性 提取 细胞毒性 

分 类 号：TQ630[化学工程—精细化工]