温敏响应水凝胶释放益生元重塑牙周菌群稳态  

Thermosensitive Hydrogel Releases Prebiotics for Remodeling Periodontal Flora Homeostasis

作　　者：薛云 王玉霞 张新歌[1] XUE Yun;WANG Yu-xia;ZHANG Xin-ge(Key Laboratory of Function Polymer Materials,Ministry of Education,Institute of Polymer Chemistry,College of Chemistry,Nankai University,Tianjin 300071,China;Department of Cariology and Endodontics,Tianjin Stomatological Hospital,School of Medicine,Nankai University,Tianjin 300041,China;Tianjin Key Laboratory of Oral Function Reconstruction,Tianjin 300041,China)

机构地区：[1]功能高分子材料教育部重点实验室,南开大学化学学院高分子化学研究所,天津300071 [2]天津市口腔医院牙体牙髓一科,南开大学医学院,天津300041 [3]天津市口腔功能重建重点实验室,天津300041

出　　处：《离子交换与吸附》2025年第2期150-155,I0003,共7页Ion Exchange and Adsorption

基　　金：国家自然科学基金(基金号21975133);天津市自然科学基金(基金号23JCYBJC01480)。

摘　　要：细菌生物被膜可诱导牙周组织产生慢性炎症,主要表现为牙周袋形成、牙槽骨吸收和牙齿松动等,严重影响人类的健康。临床治疗牙周炎主要采用机械洁牙/根面平整术、外科手术以及全身或局部施用抗菌药物等策略,但治疗效果不理想,易复发。重塑牙周菌群微环境是治疗牙周炎的一项重要措施。针对牙周治疗药物在牙周袋内滞留时间短,难以达到有效治疗效果这一问题,文章依据牙周组织结构的特征和细菌感染的病理特征,以N-乙酰-D-甘露糖胺为益生元,PLGA-PEGPLGA为载体,构建了重塑菌群稳态的温敏活性水凝胶。该活性水凝胶可适应牙周复杂的环境和不规则的形状,体外稳定释放长达8 d,延长了药物滞留时间。当N-乙酰-D-甘露糖胺的浓度为1 mol/L时,可有效促使多物种生物被膜有益菌的含量增加,高达94%,实现了菌群稳态的重塑,为细菌引起慢性炎症的治疗提供了新借鉴。Bacterial biofilms trigger chronic inflammation in periodontal tissues, primarily characterized by periodontal pocket formation, alveolar bone resorption, and tooth mobility, significantly impacting human health.Clinical treatment strategies for periodontitis mainly involve mechanical scaling/root planning, surgical procedures, and systemic or localized delivery of antimicrobial agents. However, these methods have unsatisfactory effects, leading to relapse of periodontitis. Remodeling the periodontal flora microenvironment is crucial for effective periodontitis management. This study aims to overcome the challenge posed by the brief retention time of drugs in periodontal pockets, which impedes efficacy. Leveraging the characteristics of periodontal tissue structure and bacterial infection pathology, we develop a thermosensitive active hydrogel to remodel bacterial colony homeostasis. N-acetyl-D-mannosamine serves as a probiotic, while PLGA-PEG-PLGA acts as a carrier. The active hydrogel adapts to the intricate periodontium environment, releasing the drug steadily for up to 8 days in vitro, substantially extending drug retention periods. When the concentration of N-acetyl-Dmannosamine is 1 mol/L, the active hydrogel modulated the proportion of beneficial bacteria in multi-species biofilm to 94%, showcasing remarkable remodeling of flora homeostasis.

关 键 词：温敏水凝胶 牙周炎 菌群调节 控制释放 

分 类 号：R78[医药卫生—口腔医学]