乙酰水杨酸处理对猕猴桃果实成熟衰老的影响及其作用机理  

Effects of Aspirin Treatment on Kiwifruit Ripening and Senescence and Its Mechanism

作　　者：张玉[1] 陈昆松[1] 张上隆[1] 

机构地区：[1]浙江大学果实分子生理与生物技术实验室,华家池校区杭州310029

出　　处：《植物生理与分子生物学学报》2002年第6期425-432,共8页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金 (3 0 2 70 917);浙江省自然科学基金重点项目(ZD0 0 0 4)资助

摘　　要：以不同后熟软化阶段猕猴桃果肉组织圆片为材料 ,在 2 0℃下用 1.0mmol L(pH 3.5 )的乙酰水杨酸(ASP)分别处理 4、12和 2 4h后 ,分析其对果实成熟衰老相关因子的影响。结果表明 ,随着果实成熟衰老 ,内源游离SA下降 ,LOX活性增加 ,超氧自由基 (O- ·2 )生成速率增加 ,乙烯释放量加大 ;ASP处理促使组织内源SA水平的增加 ,降低了O- ·2 生成速率 ,抑制了LOX、ACC合成酶和ACC氧化酶的活性以及乙烯的生成。推测ASP可能作为O- ·2 等自由基清除剂 ,通过负反馈调控LOX途径 。Kiwifruit [ Actinidia deliciosa C. F. Liang cv. Bruno] discs taken at different ripening stages were used to investigate the effects of acetylsalicylic acid (ASP, 1.0 mmol/L, pH 3.5) treatment on changes in endogenous salicylic acid (SA)content, LOX activity, superoxide radical (O -· 2) production rate, and ethylene biosynthesis. The results showed that the endogenous free SA declined, LOX activity, O -· 2 production rate and ethylene production increased within 24 h in the control (Figs.1-3, 5). ASP treatment elevated the endogenous SA (both conjugated and free) level, inhibited LOX activity, slowed down O -· 2 production rate, suppressed ACC synthase and ACC oxidase activities and ethylene biosynthesis (Figs.1-3, 5). The effects of ASP treatment were marked in early ripening stage and lowered in a later ripening stage (Figs.2,3,5). The significant negative correlations existed between change of endogenous SA level in the fruit discs and changes in LOX activity,O -· 2 production rate, and ACC synthase and ACC oxidase activities and ethylene production, respectively(Fig. 4). O -· 2 treatment increased LOX,AOS,ACC synthase and ACC oxidase activities and ethylene production in fruit discs(Fig.6), and the effects could be eliminated by ASP treatment (Fig.5) . It is suggested that the effects of ASP on ripening kiwifruit might be attributable to its ability to scavenge O -· 2.

关 键 词：乙酰水杨酸处理 猕猴桃 果实 成熟 衰老 作用机理 

分 类 号：S663.4[农业科学—果树学]