人参三醇对K562白血病细胞的抑制及增加化疗药敏感性的研究  

Study on the Effect of Panaxatriol on the Sensitivity of Chemical Medicine to Leukemia Cells

作　　者：金锦梅[1] 徐卫红[1] 朱泱平[1] 高瑞兰[1] 马珂[2] 

机构地区：[1]浙江省中医院血液病研究所,杭州310006 [2]浙江中医学院

出　　处：《浙江中西医结合杂志》2003年第1期10-13,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：中国和澳大利亚国际合作项目;澳方政府资助 ( IDP Education A ustralia;Phase2 - 8)国家中医药管理局重点课题 ( 972 0 2 5);浙江省中医管理局课题

摘　　要：目的 :探讨人参三醇 (GPT)对 K5 62白血病细胞生长的影响 ,及对化疗药敏感性的协同作用。方法 :在液体培养、半固体集落形成培养中 ,以不同浓度的 GPT处理 K5 62细胞 2 4、48、72小时 ,用细胞生长曲线和集落形成率来观察 GPT的增殖和抑制效应 ;用 MTT法检测 GPT对化疗药物的协同性。结果 :低浓度 (1 0μg/ml、2 0μg/ml)的 GPT能显著地刺激 K5 62细胞增殖 ,中浓度(5 0μg/ml)时使细胞增殖受抑 ,并呈时间—效应依赖性。 GPT分别与化疗药物高三尖杉酯硷(HHr)、阿糖胞苷 (Ara-c)联合应用能增加对 K5 62细胞的杀伤作用 ,抑制率为 (88.4± 2 .7) %和(73 .8± 8.8) % ,明显高于对照组的 (4 9.3± 9.3 ) %和 (3 0 .1± 7.5 ) % (P<0 .0 1 )。对低敏的足叶乙甙 (Vp-1 6)组也能使抑制率从原先的 <1 0 %提高到 >5 0 %。结论 :不同剂量的 GPT对 K5 62细胞具有刺激增殖和抑制生长的双相效应 ,并能增强 K5 62细胞对化疗药物的敏感性 ,是人参皂甙内抑制白血病细胞的有效成分 ,可为临床应用提供依据。Objective: To invistigate the effect of Panaxatrial(GPT) on the growth of K562 leukemia cells and its cooperation on the sensitivity of chemical medicine.Methods:K562 cells were dealed with different concentrations for 24h,48,72 hours in the culture of liquid,semi solid colony.And the effect of GPT's proliferation and inhibition were observed by cell growth curve and colony forming rate.The cooperation of GPT with chemical medicine was cheked by the way of MTT.Results: Low concentration (10μg/ml,20μg/ml) of GPT could stimulate the proliferation of K562 cells significantly,while middle concentration (50μg/ml) inhibited the proliferation,which showed a sort of time effect reliance.The ability of killing K562 cells could be improved in when treated by GPT together with HHr and Ara c.The rates of inhibition were 88.4±2.7% and 73.8±8.8%,which were obviously higher than those in control groups (49.3±9.3% and 30.1±7.5%)( P <0.01).The rate of inhibition in Vp 16 group with poor sensitivity could also be raised from 10% to 50%. Conclusion:Different dose of GPTs have different function,some promoting while others inhibiting the growth of K562 cells.But all can strenthen the sensitivity of K562 cells to chemical medicine.GPT is the effective component of Ginsenoside to inhibit the leukemia cells.It provides some basis for clinical practive.

关 键 词：人参三醇 K562细胞 细胞增殖 化疗药物 GPT 白血病 

分 类 号：R733.7[医药卫生—肿瘤]