细胞因子和细胞间粘附分子与酒精性肝纤维化的关系  

Relationship of Cellular Factor and Intercellular Adhesive Molecule in Ethanol-Induced Liver Fibrosis

作　　者：王炳元[1] 曹艳雪[1] 崔巍[1] 周惠敏 庞艳华 傅宝玉[1] 

机构地区：[1]中国医科大学第一临床学院消化内科,辽宁沈阳110001

出　　处：《中国医科大学学报》2002年第6期419-421,共3页Journal of China Medical University

摘　　要：目的 :应用酒精性肝病动物模型研究细胞因子和细胞间粘附分子与酒精性肝纤维化的关系。方法 :75只Wistar系雄性大鼠 ,随机分入模型组 60只和对照组 15只 ,采用酒精浓度及含量逐渐递增的方法直接给大鼠灌胃 ,制成不同病理改变的动物模型 ;用免疫组化的方法检测不同病理阶段血小板源生长因子B(PDGF B)、转化生长因子 β1(TGF β1)及细胞间粘附分子 1(ICAM 1)的表达。结果 :模型组 2 8,5 6,84d后PDGF B分别为 (67± 15 )、(13 0± 3 0 )、(2 0 2± 2 0 ) ,与对照组 (3 1± 18)kU/L相比 ,5 6和 84d均有明显性差异 (P <0 .0 5和P <0 .0 1) ;5 6d和84d后TGF β1分别为 (15 .5 2± 0 .42 )ng/ml和 (17.77± 0 .2 5 )ng/ml,与对照组 (12 .2 6± 0 .0 5 )ng/ml相比均有十分显著性差异 (P <0 .0 1) ;ICAM 1随时间推移 ,5 6d和 84d分别为 (2 .2 4± 1.0 4)和 (5 .0 8± 1.0 1) ,与对照组间也有显著性的差异 (P <0 .0 5 )。结论 :单独酒精持续性地刺激大鼠 ,细胞因子和细胞间粘附分子等进行性增加的同时 。Objective: We studied the gene expressions of cellular growth factor and intercellular adhesion molecule in ethanol induced liver fibrosis. Methods: We established a rat model of alcoholic liver disease by direct intragastric inoculation of ethanol. Seventy five Wistar rats weighting 160 to 200 grams were randomized to control (15 rats) and model (60 rats) groups. Immunochemical stainings for the expressions of platelet derived growth factor B (PDGF B), transforming growth factor β 1 (TGF β 1), and intercellular adhesion molecule (ICAM 1) were performed. Results: There was a marked increase in expression of PDGF B (67±15)kU·L -1 , (130±30)kU·L -1 , (202±20)kU·L -1 at the 28 th, 56 th, and 84 th dayrespectivelyucomparedwiththecontrolgroup(31±18 kU·L -1 , P<0.05), andTGF β 1(15.52±0.42) ng/mland (17.77±0.25)ng/ml at the 56 th and 84 th day, respectively compared with the control group (12.26±0.05ng/ml, P<0.01) in the ethanol fed rats. The levels increased further with prolonged alcohol feeding (P<0.01). In contrast, ICAM 1 level increased only at the 28th day and correlated with both the amount and the duration of alcohol feeding thereafter (2.24±1.04 and 5.08±1.01) at the 56th and 84th day, respectively (P<0.05). Conclusion: Both cellular growth factors and ICAM increase progressively with ethanol-induced liver injury as liver fibrosis develops concomitantly.

关 键 词：细胞因子 细胞间粘附分子 酒精性肝纤维化 血小板源生长因子 转化生长因子 

分 类 号：R575.2[医药卫生—消化系统]